Global Patent Index - EP 1025234 A1

EP 1025234 A1 2000-08-09 - BINARY VIRAL EXPRESSION SYSTEM IN PLANTS

Title (en)

BINARY VIRAL EXPRESSION SYSTEM IN PLANTS

Title (de)

BINÄRE VIRALE EXPRESSIONSSYSTEME IN PFLANZEN

Title (fr)

SYSTEME BINAIRE D'EXPRESSION VIRALE DANS DES PLANTES

Publication

EP 1025234 A1 (EN)

Application

EP 98953997 A

Priority

  • US 9822688 W
  • US 6350497 P
  • US 10155898 P

Abstract (en)

[origin: WO9922003A1] This invention relates to a regulated binary plant viral expression system. It is comprised of two chromosomally-integrated components. One component is a proreplicon, which contains cis-acting viral sequences required for replication and a contains a target gene. The other component is a chimeric trans-acting replication gene comprising a regulated promoter operably-linked to the coding region for a viral replication protein. The proreplicon lacks the replication gene essential for replicon replication, and thus cannot undergo autonomous episomal replication. However, regulated expression of the trans-acting replication protein in plant cells also containing the proreplicon will trigger the release of free replicon from the integrated proreplicon, result in its episomal replication in trans, and result in the expression of the target gene, if present, through gene amplification. The expression system is useful for both production of foreign proteins as well as silencing endogenous genes and transgenes in plant tissue. Tissue-specific expression is controlled by the choice of promoter controlling the transcription of the trans-acting replication gene.

IPC 1-7 (main, further and additional classification)

C12N 15/34; C12N 15/82

IPC 8 full level (invention and additional information)

C07K 14/01 (2006.01); C12N 15/34 (2006.01); C12N 15/82 (2006.01)

CPC (invention and additional information)

C07K 14/005 (2013.01); C12N 15/8203 (2013.01); C12N 15/8216 (2013.01); C12N 15/8217 (2013.01); C12N 15/8238 (2013.01); C12N 2750/12022 (2013.01)

Citation (search report)

See references of WO 9922003A1

Designated contracting state (EPC)

DE ES FR GB IT SE

EPO simple patent family

WO 9922003 A1 19990506; AU 1122599 A 19990517; AU 731330 B2 20010329; BR 9815260 A 20001121; CA 2304984 A1 19990506; EP 1025234 A1 20000809; HU 0004222 A2 20010328; HU 0004222 A3 20021128; PL 340297 A1 20010129

INPADOC legal status


2004-10-13 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20040407

2000-08-09 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20000317

2000-08-09 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): DE ES FR GB IT SE