EP 1025234 A1 20000809 - BINARY VIRAL EXPRESSION SYSTEM IN PLANTS
Title (en)
BINARY VIRAL EXPRESSION SYSTEM IN PLANTS
Title (de)
BINÄRE VIRALE EXPRESSIONSSYSTEME IN PFLANZEN
Title (fr)
SYSTEME BINAIRE D'EXPRESSION VIRALE DANS DES PLANTES
Publication
Application
Priority
- US 9822688 W 19981023
- US 6350497 P 19971024
- US 10155898 P 19980923
Abstract (en)
[origin: WO9922003A1] This invention relates to a regulated binary plant viral expression system. It is comprised of two chromosomally-integrated components. One component is a proreplicon, which contains cis-acting viral sequences required for replication and a contains a target gene. The other component is a chimeric trans-acting replication gene comprising a regulated promoter operably-linked to the coding region for a viral replication protein. The proreplicon lacks the replication gene essential for replicon replication, and thus cannot undergo autonomous episomal replication. However, regulated expression of the trans-acting replication protein in plant cells also containing the proreplicon will trigger the release of free replicon from the integrated proreplicon, result in its episomal replication in trans, and result in the expression of the target gene, if present, through gene amplification. The expression system is useful for both production of foreign proteins as well as silencing endogenous genes and transgenes in plant tissue. Tissue-specific expression is controlled by the choice of promoter controlling the transcription of the trans-acting replication gene.
IPC 1-7
IPC 8 full level
C07K 14/01 (2006.01); C12N 15/34 (2006.01); C12N 15/82 (2006.01)
CPC (source: EP)
C07K 14/005 (2013.01); C12N 15/8203 (2013.01); C12N 15/8216 (2013.01); C12N 15/8217 (2013.01); C12N 15/8238 (2013.01); C12N 2750/12022 (2013.01)
Designated contracting state (EPC)
DE ES FR GB IT SE
DOCDB simple family (publication)
WO 9922003 A1 19990506; AU 1122599 A 19990517; AU 731330 B2 20010329; BR 9815260 A 20001121; CA 2304984 A1 19990506; EP 1025234 A1 20000809; HU P0004222 A2 20010328; HU P0004222 A3 20021128; PL 340297 A1 20010129
DOCDB simple family (application)
US 9822688 W 19981023; AU 1122599 A 19981023; BR 9815260 A 19981023; CA 2304984 A 19981023; EP 98953997 A 19981023; HU P0004222 A 19981023; PL 34029798 A 19981023