EP 1029077 A2 20000823 - A SPECIFIC AND SENSITIVE NULCEIC ACID DETECTION METHOD
Title (en)
A SPECIFIC AND SENSITIVE NULCEIC ACID DETECTION METHOD
Title (de)
SPEZIFISCHES UND EMPFINDLICHES NUKLEINSÄURENACHWEISVERFAHREN
Title (fr)
PROCEDE SPECIFIQUE ET SENSIBLE POUR LA DETECTION D'ACIDES NUCLEIQUES
Publication
Application
Priority
- DE 19748690 A 19971104
- DE 19814001 A 19980328
- DE 19814828 A 19980402
- EP 9806952 W 19981103
Abstract (en)
[origin: WO9923250A2] The invention relates to a method for detecting a nucleic acid, comprising the following steps: the production of a number of enhancers of a fragment of said nucleic acid with a length of less than 100 nucleotides using two primers, one of said primers being able to bind to a first binding sequence (A) of a strand of the nucleic acid and the other primer being able to bind to a second binding sequence (C') which is essentially complementary to a sequence (C), said sequence (C) not overlapping with (A) and being positioned in the direction 3' from A, in the presence of a probe with a binding sequence (D) which is able to bind to the third sequence (B) positioned between the sequences (A) and (C) or the complement (B') thereof, said probe containing a reporter group and a quencher group, using a polymerase with 5' nuclease activity; and detection of the nucleic acid through the measurement of a signal which is determined by the release of the reporter group.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP US)
C12Q 1/6818 (2013.01 - EP US); C12Q 1/6823 (2013.01 - EP US); C12Q 1/686 (2013.01 - EP US)
C-Set (source: EP US)
Citation (examination)
- US 5453355 A 19950926 - BIRKENMEYER LARRY G [US], et al
- WO 9859072 A1 19981230 - AFFYMETRIX INC [US], et al
- WOLFF C; HÖRNSCHEMEYER D; SKURTOPULOS M; PETERSEN N; BEYER J; SEIDEL K; MUTZ A; KLEESIEK K: "Molecular biological screening of viruses important to transfusion medicine", BEITRÄGE ZUR INFUSIONSTHERAPIE UND TRANSFUSIONSMEDIZIN = CONTRIBUTIONS TO INFUSION THERAPY AND TRANSFUSION MEDICINE, vol. 32, 1994, Switzerland, pages 102 - 109
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB IE IT LI LU NL SE
DOCDB simple family (publication)
WO 9923250 A2 19990514; WO 9923250 A3 19990722; AU 1232099 A 19990524; AU 2152099 A 19990531; AU 2152199 A 19990524; AU 741141 B2 20011122; CA 2308368 A1 19990514; CA 2308368 C 20090120; CA 2308762 A1 19990514; CA 2312779 A1 19990520; EP 1029077 A2 20000823; EP 1029083 A2 20000823; EP 1029084 A2 20000823; JP 2001521765 A 20011113; JP 2002505071 A 20020219; JP 2002509694 A 20020402; US 2003175765 A1 20030918; US 7105318 B2 20060912; WO 9923249 A2 19990514; WO 9923249 A3 19990910; WO 9924606 A2 19990520; WO 9924606 A3 19990722
DOCDB simple family (application)
EP 9806961 W 19981103; AU 1232099 A 19981103; AU 2152099 A 19981103; AU 2152199 A 19981103; CA 2308368 A 19981103; CA 2308762 A 19981103; CA 2312779 A 19981103; EP 9806951 W 19981103; EP 9806952 W 19981103; EP 98955529 A 19981103; EP 98965652 A 19981103; EP 98965653 A 19981103; JP 2000519104 A 19981103; JP 2000519105 A 19981103; JP 2000519599 A 19981103; US 32213802 A 20021217