EP 1029084 A2 20000823 - SPECIFIC AND SENSITIVE METHOD FOR DETECTING NUCLEIC ACIDS
Title (en)
SPECIFIC AND SENSITIVE METHOD FOR DETECTING NUCLEIC ACIDS
Title (de)
SPEZIFISCHES UND SENSITIVES NUKLEINSÄURENACHWEISVERFAHREN
Title (fr)
PROCEDE SPECIFIQUE ET SENSIBLE POUR LA DETECTION D'ACIDE NUCLEIQUE
Publication
Application
Priority
- DE 19748690 A 19971104
- DE 19814001 A 19980328
- DE 19814828 A 19980402
- EP 9806961 W 19981103
Abstract (en)
[origin: WO9923250A2] The invention relates to a method for detecting a nucleic acid, comprising the following steps: the production of a number of enhancers of a fragment of said nucleic acid with a length of less than 100 nucleotides using two primers, one of said primers being able to bind to a first binding sequence (A) of a strand of the nucleic acid and the other primer being able to bind to a second binding sequence (C') which is essentially complementary to a sequence (C), said sequence (C) not overlapping with (A) and being positioned in the direction 3' from A, in the presence of a probe with a binding sequence (D) which is able to bind to the third sequence (B) positioned between the sequences (A) and (C) or the complement (B') thereof, said probe containing a reporter group and a quencher group, using a polymerase with 5' nuclease activity; and detection of the nucleic acid through the measurement of a signal which is determined by the release of the reporter group.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP US)
C12Q 1/6818 (2013.01 - EP US); C12Q 1/6823 (2013.01 - EP US); C12Q 1/686 (2013.01 - EP US)
Citation (search report)
See references of WO 9923250A2
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB IE IT LI LU NL SE
DOCDB simple family (publication)
WO 9923250 A2 19990514; WO 9923250 A3 19990722; AU 1232099 A 19990524; AU 2152099 A 19990531; AU 2152199 A 19990524; AU 741141 B2 20011122; CA 2308368 A1 19990514; CA 2308368 C 20090120; CA 2308762 A1 19990514; CA 2312779 A1 19990520; EP 1029077 A2 20000823; EP 1029083 A2 20000823; EP 1029084 A2 20000823; JP 2001521765 A 20011113; JP 2002505071 A 20020219; JP 2002509694 A 20020402; US 2003175765 A1 20030918; US 7105318 B2 20060912; WO 9923249 A2 19990514; WO 9923249 A3 19990910; WO 9924606 A2 19990520; WO 9924606 A3 19990722
DOCDB simple family (application)
EP 9806961 W 19981103; AU 1232099 A 19981103; AU 2152099 A 19981103; AU 2152199 A 19981103; CA 2308368 A 19981103; CA 2308762 A 19981103; CA 2312779 A 19981103; EP 9806951 W 19981103; EP 9806952 W 19981103; EP 98955529 A 19981103; EP 98965652 A 19981103; EP 98965653 A 19981103; JP 2000519104 A 19981103; JP 2000519105 A 19981103; JP 2000519599 A 19981103; US 32213802 A 20021217