Global Patent Index - EP 1032659 A1

EP 1032659 A1 2000-09-06 - GRAFTED COPOLYMERS AS GENE CARRIERS

Title (en)

GRAFTED COPOLYMERS AS GENE CARRIERS

Title (de)

GEPFROPFTE COPOLYMERE ALS GENTRÄGER

Title (fr)

COPOLYMERES GREFFES UTILISES COMME PORTEURS DE GENES

Publication

EP 1032659 A1 (EN)

Application

EP 98963119 A

Priority

  • US 9826451 W
  • US 6935197 P

Abstract (en)

[origin: WO9929839A1] A non-toxic, polymeric gene carrier used to deliver a selected nucleic acid into a host cell comprises a grafted copolymer having straight-chain amphiphilic side polymers grafted to a polycationic main polymer. The polycationic main polymer forms a complex with nucleic acids by electrostatic interaction. The amphiphilic side polymers reduce the cytoxicity of the polycation and enhance solubility. The molar proportion of side polymers may be adjusted to optimize transfection efficiency. Likewise, the ratio of nucleic acid to copolymer may be adjusted to optimize efficiency. The composition may be used in a method for transforming a host cell with a selected nucleic acid. Grafted copolymers of polyethylene glycol and poly-L-lysine (PEG-g-PLL) are particularly effective, achieving peak gene expression within 24 hours and persisting for at least 96 hours.

IPC 1-7 (main, further and additional classification)

C12N 15/00; A01N 63/00; A61K 48/00

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); A61K 31/4709 (2006.01); A61K 45/00 (2006.01); A61K 47/48 (2006.01); A61K 48/00 (2006.01); C12N 15/87 (2006.01)

CPC (invention and additional information)

A61K 48/00 (2013.01); C12N 15/87 (2013.01)

Citation (search report)

See references of WO 9929839A1

Designated contracting state (EPC)

AT BE CH DE DK ES FI FR GB GR IE IT LI NL SE

EPO simple patent family

WO 9929839 A1 19990617; AR 013017 A1 20001122; AU 1820999 A 19990628; BR 9814273 A 20001003; EP 1032659 A1 20000906; IL 136679 D0 20010614; JP 2001526181 A 20011218; ZA 9811378 B 19990805

INPADOC legal status


2002-01-30 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20010817

2000-09-06 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20000608

2000-09-06 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH DE DK ES FI FR GB GR IE IT LI NL SE