EP 1032703 A1 20000906 - HETEROZYGOTE SCREENING PROCESS
Title (en)
HETEROZYGOTE SCREENING PROCESS
Title (de)
NACHWEISVERFAHREN HETEROZYGOTER GENLOCI
Title (fr)
PROCEDE DE CRIBLAGE D'HETEROZYGOTES
Publication
Application
Priority
- GB 9803541 W 19981119
- GB 9724480 A 19971119
Abstract (en)
[origin: WO9925872A1] In order to facilitate the screening of an organism, or a population of organisms, carrying heterozygous mutations for identifying the presence of a mutation in a gene of interest, a process is provided which utilises mismatch binding proteins, such as MutS. The process comprises the steps of: obtaining a nucleic acid sample from the organism; denaturing any double stranded nucleic acid present in the sample; allowing the nucleic acid to anneal; and removing homoduplexes from the annealed sample. A positive signal from a probe specific for the gene of interest indicates that the organism carries a heterozygous mutation in said gene.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6809 (2018.01)
CPC (source: EP)
C12Q 1/6809 (2013.01); C12Q 2600/156 (2013.01)
Citation (search report)
See references of WO 9925872A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9925872 A1 19990527; AU 1251899 A 19990607; CA 2311526 A1 19990527; EP 1032703 A1 20000906; GB 9724480 D0 19980114; IL 136228 A0 20010520; JP 2002514382 A 20020521
DOCDB simple family (application)
GB 9803541 W 19981119; AU 1251899 A 19981119; CA 2311526 A 19981119; EP 98955793 A 19981119; GB 9724480 A 19971119; IL 13622898 A 19981119; JP 2000521235 A 19981119