Global Patent Index - EP 1032703 A1

EP 1032703 A1 2000-09-06 - HETEROZYGOTE SCREENING PROCESS

Title (en)

HETEROZYGOTE SCREENING PROCESS

Title (de)

NACHWEISVERFAHREN HETEROZYGOTER GENLOCI

Title (fr)

PROCEDE DE CRIBLAGE D'HETEROZYGOTES

Publication

EP 1032703 A1 (EN)

Application

EP 98955793 A

Priority

  • GB 9803541 W
  • GB 9724480 A

Abstract (en)

[origin: WO9925872A1] In order to facilitate the screening of an organism, or a population of organisms, carrying heterozygous mutations for identifying the presence of a mutation in a gene of interest, a process is provided which utilises mismatch binding proteins, such as MutS. The process comprises the steps of: obtaining a nucleic acid sample from the organism; denaturing any double stranded nucleic acid present in the sample; allowing the nucleic acid to anneal; and removing homoduplexes from the annealed sample. A positive signal from a probe specific for the gene of interest indicates that the organism carries a heterozygous mutation in said gene.

IPC 1-7 (main, further and additional classification)

C12Q 1/68

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); C12Q 1/68 (2006.01)

CPC (invention and additional information)

C12Q 1/6809 (2013.01); C12Q 2600/156 (2013.01)

Combination set (CPC)

C12Q 1/6809 + C12Q 2522/101

Citation (search report)

See references of WO 9925872A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9925872 A1 19990527; AU 1251899 A 19990607; CA 2311526 A1 19990527; EP 1032703 A1 20000906; GB 9724480 D0 19980114; IL 136228 D0 20010520; JP 2002514382 A 20020521

INPADOC legal status


2003-08-06 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20030129

2002-09-04 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20020718

2000-09-06 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20000602

2000-09-06 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE