Global patent index - EP 1034272 A1

EP 1034272 A1 2000-09-13 - A cDNA ENCODING C6 $g(b)-CHEMOKINE LEUKOTACTIN-1(Lkn-1) ISOLATED FROM HUMAN

Title (en)

A cDNA ENCODING C6 $g(b)-CHEMOKINE LEUKOTACTIN-1(Lkn-1) ISOLATED FROM HUMAN

Title (de)

EINE AUS DEM MENSCHEN ISOLIERTE cDNA, DIE FÜR C6-BETA-CHEMOKIN LEUKOTAKTIN-1 (LKN-1) KODIERT

Title (fr)

ADNc CODANT UNE C6 $g(b)-CHEMOKINE LEUKOTACTINE-1 (Lkn-1) ISOLEE D'UN ORGANISME HUMAIN

Publication

EP 1034272 A1 (EN)

Application

EP 98959235 A

Priority

  • KR 9800381 W
  • KR 19970063610 A

Abstract (en)

[origin: WO9928473A1] The present invention relates to a cDNA coding for a novel protein which belongs to C6 beta -chemokines and attracts subsets of peripheral blood leukocytes, and a process for preparing the said protein by employing expression vector therefor. Open reading frame of the Lkn-1 cDNA encodes 113 amino acids containing a signal peptide of 21 amino acids, and molecular weight of mature protein consisting of 92 amino acids among them is supposed to be 10,162 dalton. A recombinant Lkn-1 which was expressed in E. coli or insect cell employing the said Lkn-1 cDNA and purified, inhibited colony formation and cell proliferation significantly, attracted neutrophils, monocytes and lymphocytes to cause chemotaxis, and bound to CCR1 and CCR3 receptors. Accordingly, it was determined that the recombinant Lkn-1 protein can be used as a potential drug for antibody production, the treatment during HIV-1 infection, the protection of bone marrow stem cells during chemotherapy or radiotherapy and the inhibition of leukemia, etc.

IPC 1-7 (main, further and additional classification)

C12N 15/19; A61K 38/19; C07K 14/52; C12N 5/10

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); A61K 38/00 (2006.01); A61P 37/02 (2006.01); A61P 43/00 (2006.01); C07K 14/52 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 15/12 (2006.01); C12N 15/19 (2006.01)

CPC (invention and additional information)

C07K 14/523 (2013.01); A61K 38/00 (2013.01)

Citation (search report)

See references of WO 9928473A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9928473 A1 19990610; CN 1282371 A 20010131; EP 1034272 A1 20000913; JP 2002505076 A 20020219; KR 100367213 B1 20030109; KR 20010022352 A 20010315; WO 9928472 A1 19990610

INPADOC legal status

2003-10-01 [18D] DEEMED TO BE WITHDRAWN

- Ref Legal Event Code: 18D

- Effective date: 20030325

2003-01-02 [17Q] FIRST EXAMINATION REPORT

- Ref Legal Event Code: 17Q

- Effective date: 20021112

2000-09-20 [17P] REQUEST FOR EXAMINATION FILED

- Ref Legal Event Code: 17P

- Effective date: 20000621

2000-09-13 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE