EP 1034285 A2 20000913 - ISOLATION AND CHARACTERIZATION OF PLANT REGULATORY SEQUENCES
Title (en)
ISOLATION AND CHARACTERIZATION OF PLANT REGULATORY SEQUENCES
Title (de)
ISOLATION UND CHARAKTERISIERUNG VON REGULATORISCHEN SEQUENZEN AUS PFANZEN
Title (fr)
ISOLATION ET CARACTERISATION DE SEQUENCES REGULATRICES DE PLANTES
Publication
Application
Priority
- EP 98966826 A 19981126
- EP 9807761 W 19981126
- EP 97203701 A 19971127
Abstract (en)
[origin: WO9928483A2] In the search for plant regulatory sequences capable of driving nematode-triggered effector gene expression in feeding structures the promoter tagging appears to be a valuable tool. A large collection of transgenic Arabidopsis thaliana(L.) plants was generated. They were transformed with a beta -glucuronidase gene functioning as a promoter tag. Two T-DNA constructs, pGV1047 and p DELTA gusBin19 were used. Early responses to nematode invasion were of primary interest. Four lines exhibiting GUS activity in syncytia induced by the beet cyst nematode were studied. Reporter gene activation was also identified in galls induced by root knot and ectoparasitic nematodes. Time course studies revealed that the four tags were differentially activated during the development of the feeding structure. T-DNA-flanking regions responsible for the observed responses after nematode infection were isolated and characterized for promoter activity.
IPC 1-7
C12N 15/82; C12N 15/29; C12N 5/10; C12N 9/22; A01H 5/00; A01N 65/00
IPC 8 full level
A01H 5/00 (2006.01); A01N 65/00 (2006.01); A01N 65/08 (2009.01); C12N 5/10 (2006.01); C12N 9/22 (2006.01); C12N 15/29 (2006.01); C12N 15/82 (2006.01)
CPC (source: EP)
A01N 65/00 (2013.01); A01N 65/08 (2013.01); C12N 15/8237 (2013.01); C12N 15/8239 (2013.01); C12N 15/8285 (2013.01); Y02A 40/146 (2017.12)
Citation (search report)
See references of WO 9928483A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9928483 A2 19990610; WO 9928483 A3 19990722; AU 2513199 A 19990616; AU 748761 B2 20020613; CA 2311571 A1 19990610; EP 1034285 A2 20000913
DOCDB simple family (application)
EP 9807761 W 19981126; AU 2513199 A 19981126; CA 2311571 A 19981126; EP 98966826 A 19981126