Global Patent Index - EP 1034285 A2

EP 1034285 A2 2000-09-13 - ISOLATION AND CHARACTERIZATION OF PLANT REGULATORY SEQUENCES

Title (en)

ISOLATION AND CHARACTERIZATION OF PLANT REGULATORY SEQUENCES

Title (de)

ISOLATION UND CHARAKTERISIERUNG VON REGULATORISCHEN SEQUENZEN AUS PFANZEN

Title (fr)

ISOLATION ET CARACTERISATION DE SEQUENCES REGULATRICES DE PLANTES

Publication

EP 1034285 A2 (EN)

Application

EP 98966826 A

Priority

  • EP 98966826 A
  • EP 9807761 W
  • EP 97203701 A

Abstract (en)

[origin: WO9928483A2] In the search for plant regulatory sequences capable of driving nematode-triggered effector gene expression in feeding structures the promoter tagging appears to be a valuable tool. A large collection of transgenic Arabidopsis thaliana(L.) plants was generated. They were transformed with a beta -glucuronidase gene functioning as a promoter tag. Two T-DNA constructs, pGV1047 and p DELTA gusBin19 were used. Early responses to nematode invasion were of primary interest. Four lines exhibiting GUS activity in syncytia induced by the beet cyst nematode were studied. Reporter gene activation was also identified in galls induced by root knot and ectoparasitic nematodes. Time course studies revealed that the four tags were differentially activated during the development of the feeding structure. T-DNA-flanking regions responsible for the observed responses after nematode infection were isolated and characterized for promoter activity.

IPC 1-7 (main, further and additional classification)

C12N 15/82; A01H 5/00; A01N 65/00; C12N 5/10; C12N 9/22; C12N 15/29

IPC 8 full level (invention and additional information)

A01H 5/00 (2006.01); A01N 65/08 (2009.01); C12N 5/10 (2006.01); C12N 9/22 (2006.01); C12N 15/29 (2006.01); C12N 15/82 (2006.01)

CPC (invention and additional information)

A01N 65/08 (2013.01); A01N 65/00 (2013.01); C12N 15/8237 (2013.01); C12N 15/8239 (2013.01); C12N 15/8285 (2013.01)

Combination set (CPC)

A01N 65/00 + A01N 2300/00

Citation (search report)

See references of WO 9928483A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9928483 A2 19990610; WO 9928483 A3 19990722; AU 2513199 A 19990616; AU 748761 B2 20020613; CA 2311571 A1 19990610; EP 1034285 A2 20000913

INPADOC legal status


2004-05-19 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20031112

2003-08-13 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20030701

2000-09-13 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20000511

2000-09-13 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE