Global Patent Index - EP 1034300 A1

EP 1034300 A1 2000-09-13 - TETRACYCLINE ASSAY METHOD

Title (en)

TETRACYCLINE ASSAY METHOD

Title (de)

VERFAHREN ZUR BESTIMMUNG VON TETRAZYKLINEN

Title (fr)

PROCEDE DE DOSAGE DE TETRACYCLINE

Publication

EP 1034300 A1 (EN)

Application

EP 98952788 A

Priority

  • FI 9800873 W
  • FI 974235 A

Abstract (en)

[origin: WO9925866A1] The invention relates to a method for the determination of a tetracycline in a sample. The method is characterized in that the sample is brought into contact with prokaryotic cells encompassing a DNA vector including a nucleotide sequence encoding a light producing enzyme under transcriptional control of a tetracycline repressor and a tetracycline promoter, detecting the luminescence emitted from the cells, and comparing the emitted luminescence to the luminescence emitted from cells in a control containing no tetracycline. The invention also concerns recombinant prokaryotic cells capable of emitting light in response to the existence of a tetracycline in a sample. Furthermore, the invention relates to novel DNA vectors useful for the construction of said prokaryotic cells.

IPC 1-7 (main, further and additional classification)

C12Q 1/66; C12N 1/21; C12N 15/53; C12Q 1/18

IPC 8 full level (invention and additional information)

C12N 9/02 (2006.01); C12N 15/53 (2006.01)

CPC (invention and additional information)

C12N 9/0069 (2013.01)

Citation (search report)

See references of WO 9925866A1

Designated contracting state (EPC)

BE DE DK ES FI FR GB IT NL SE

EPO simple patent family

WO 9925866 A1 19990527; EP 1034300 A1 20000913; FI 974235 A0 19971114

INPADOC legal status


2004-05-12 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20031104

2003-08-06 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20030623

2000-09-13 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20000518

2000-09-13 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): BE DE DK ES FI FR GB IT NL SE