EP 1034300 A1 20000913 - TETRACYCLINE ASSAY METHOD
Title (en)
TETRACYCLINE ASSAY METHOD
Title (de)
VERFAHREN ZUR BESTIMMUNG VON TETRAZYKLINEN
Title (fr)
PROCEDE DE DOSAGE DE TETRACYCLINE
Publication
Application
Priority
- FI 9800873 W 19981111
- FI 974235 A 19971114
Abstract (en)
[origin: WO9925866A1] The invention relates to a method for the determination of a tetracycline in a sample. The method is characterized in that the sample is brought into contact with prokaryotic cells encompassing a DNA vector including a nucleotide sequence encoding a light producing enzyme under transcriptional control of a tetracycline repressor and a tetracycline promoter, detecting the luminescence emitted from the cells, and comparing the emitted luminescence to the luminescence emitted from cells in a control containing no tetracycline. The invention also concerns recombinant prokaryotic cells capable of emitting light in response to the existence of a tetracycline in a sample. Furthermore, the invention relates to novel DNA vectors useful for the construction of said prokaryotic cells.
IPC 1-7
IPC 8 full level
C12N 9/02 (2006.01); C12N 15/53 (2006.01)
CPC (source: EP)
C12N 9/0069 (2013.01)
Citation (search report)
See references of WO 9925866A1
Designated contracting state (EPC)
BE DE DK ES FI FR GB IT NL SE
DOCDB simple family (publication)
WO 9925866 A1 19990527; EP 1034300 A1 20000913; FI 974235 A0 19971114
DOCDB simple family (application)
FI 9800873 W 19981111; EP 98952788 A 19981111; FI 974235 A 19971114