Global Patent Index - EP 1034304 A1

EP 1034304 A1 2000-09-13 - METHOD FOR MEASURING THE APOPTOSIS

Title (en)

METHOD FOR MEASURING THE APOPTOSIS

Title (de)

VERFAHREN ZUR MESSUNG DER APOPTOSE

Title (fr)

PROCEDE DE MESURE DE L'APOPTOSE

Publication

EP 1034304 A1 (DE)

Application

EP 98959893 A

Priority

  • DE 19752922 A
  • DE 19805229 A
  • EP 9807682 W

Abstract (en)

[origin: WO9928499A1] The invention relates to a method for quickly and easily measuring the apoptosis. Mammal cells are co-transfected with a plasmid coded for a fluorescent protein, and with a plasmid carrying an interested gene. The apoptosis is measured after incubation and mild fixation in which the DNA content of the cells is determined by means of DNA-binding colorants, and the portion of the transfected cells is determined by means of flow-through cytometry. The method can be used in order to identify substances which modulate the proapoptotic or antiapoptotic effect of genes or gene products.

IPC 1-7 (main, further and additional classification)

C12Q 1/68; G01N 33/50

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); C12Q 1/68 (2006.01); G01N 33/50 (2006.01)

CPC (invention and additional information)

G01N 33/5011 (2013.01); C12Q 1/6897 (2013.01); G01N 33/5008 (2013.01); G01N 33/5091 (2013.01); G01N 2510/00 (2013.01)

Citation (search report)

See references of WO 9928499A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9928499 A1 19990610; CA 2311954 A1 19990610; EP 1034304 A1 20000913; JP 2001525182 A 20011211

INPADOC legal status


2005-12-21 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20050601

2000-09-27 [RAP1] TRANSFER OF RIGHTS OF AN EP PUBLISHED APPLICATION

- Owner name: BOEHRINGER INGELHEIM INTERNATIONAL GMBH

2000-09-13 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20000628

2000-09-13 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE