EP 1036321 A1 20000920 - METHOD AND CELLS TO SCREEN COMBINATORIAL LIBRARIES
Title (en)
METHOD AND CELLS TO SCREEN COMBINATORIAL LIBRARIES
Title (de)
VERFAHREN UND ZELLEN ZUM "SCREENING" VON "COMBINATORIAL LIBRARIES"
Title (fr)
TECHNIQUE DE CELLULES POUR CRIBLAGE DE BIBLIOTHEQUES COMBINATOIRES
Publication
Application
Priority
- EP 98963539 A 19981207
- EP 9807922 W 19981207
- EP 97121451 A 19971205
- EP 98890217 A 19980723
Abstract (en)
[origin: WO9930151A1] This invention is a method for identifying and isolating at least one of the following: a polypeptide of interest and/or a gene thereof; a target compound and/or a gene thereof, wherein the polypeptide of interest, or the target compound, or both are unknown. The method is based on the steps of: (1) displaying a library of a polypeptide of interest on the surface of a population of prokaryotic or eukaryotic cells; (2) exposing the population to a target compound; and (3) exposing the population to a selection agent. Interaction among the three components "imprints" the polypeptide: that is, the interaction selects for those cells in which the polypeptide interacts with the target compound in a prescribed manner.
IPC 1-7
IPC 8 full level
A61K 45/00 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 11/16 (2006.01); C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12Q 1/68 (2006.01); C40B 30/04 (2006.01); C40B 40/02 (2006.01); G01N 33/15 (2006.01); G01N 33/48 (2006.01); G01N 33/50 (2006.01); G01N 33/569 (2006.01); G01N 33/68 (2006.01)
CPC (source: EP)
C12N 15/1037 (2013.01); C12N 15/1055 (2013.01); C40B 30/04 (2013.01); C40B 40/02 (2013.01); G01N 33/5008 (2013.01); G01N 33/5014 (2013.01); G01N 33/502 (2013.01); G01N 33/56916 (2013.01); G01N 33/6845 (2013.01)
Citation (search report)
See references of WO 9930151A1
Designated contracting state (EPC)
AT BE CH DE FR GB IT LI NL SE
DOCDB simple family (publication)
WO 9930151 A1 19990617; AU 1876999 A 19990628; AU 749042 B2 20020620; CA 2310381 A1 19990617; EP 1036321 A1 20000920; JP 2001526054 A 20011218
DOCDB simple family (application)
EP 9807922 W 19981207; AU 1876999 A 19981207; CA 2310381 A 19981207; EP 98963539 A 19981207; JP 2000524659 A 19981207