EP 1038012 A2 20000927 - METHOD FOR PRODUCING TRANSGENIC PLANTS WITH MODIFIED 5-AMINOLEVULINIC ACID BIOSYNTHESIS, METHOD FOR IDENTIFYING 5-AMINOLEVULINIC ACID SYNTHESIS EFFECTORS
Title (en)
METHOD FOR PRODUCING TRANSGENIC PLANTS WITH MODIFIED 5-AMINOLEVULINIC ACID BIOSYNTHESIS, METHOD FOR IDENTIFYING 5-AMINOLEVULINIC ACID SYNTHESIS EFFECTORS
Title (de)
VERFAHREN ZUR HERSTELLUNG TRANSGENER PFLANZEN MIT VERÄNDERTER 5-AMINOLÄVULINSÄURE-BIOSYNTHESE UND VERFAHREN ZUR IDENTIFIZIERUNG VON EFFEKTOREN DER 5-AMINOLÄVULINSÄURE-SYNTHESE
Title (fr)
PROCEDE DE PRODUCTION DE PLANTES TRANSGENIQUES PAR BIOSYNTHESE MODIFIEE D'ACIDE 5-AMINOLEVULINIQUE, ET PROCEDE D'IDENTIFICATION D'EFFECTEURS DE LA SYNTHESE D'ACIDE 5-AMINOLEVULINIQUE
Publication
Application
Priority
- DE 19754929 A 19971210
- EP 9808028 W 19981210
Abstract (en)
[origin: DE19754929A1] The invention relates to a method for producing transgenic plants by means of modified 5-aminolevulinic acid biosynthesis, transgenic plant cells, transgenic plant parts, transgenic seeds, and transgenic reproduction material. The invention is characterised in that one or several nucleic acid molecules coding for a protein with a 5-aminolevulinic acid synthase function (ALAS), selected from a group of feedback regulated ALAS, animal ALAS and bacterial ALAS, an active fragment thereof or an antisense or complementary sequence thereof, are integrated into the plant genome in a stable form. Effectors of natural 5-aminolevulinic acid biosynthesis in plants are found using a method for determining effector efficacy of a test substance in relation to a GSAAT, by: a) determining the enzymatic activity of the GSAAT in the absence of a test substance b) determining the enzymatic activity of the GSAAT in the presence of a test substance; and c) by comparing the enzymatic activities determined under a) and b).
IPC 1-7
IPC 8 full level
A01H 5/00 (2006.01); C12N 5/10 (2006.01); C12N 9/10 (2006.01); C12N 9/90 (2006.01); C12N 15/09 (2006.01); C12N 15/31 (2006.01); C12N 15/60 (2006.01); C12N 15/82 (2006.01); G01N 33/15 (2006.01); G01N 33/50 (2006.01)
CPC (source: EP US)
C12N 9/1029 (2013.01 - EP US); C12N 9/90 (2013.01 - EP US); C12N 15/8243 (2013.01 - EP US); C12N 15/8274 (2013.01 - EP US)
Citation (search report)
See references of WO 9929880A2
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IT LI NL SE
DOCDB simple family (publication)
DE 19754929 A1 19990617; AU 1966999 A 19990628; CA 2313706 A1 19990617; EP 1038012 A2 20000927; JP 2001526041 A 20011218; US 2003204873 A1 20031030; US 6603062 B1 20030805; WO 9929880 A2 19990617; WO 9929880 A3 19991007
DOCDB simple family (application)
DE 19754929 A 19971210; AU 1966999 A 19981210; CA 2313706 A 19981210; EP 9808028 W 19981210; EP 98964495 A 19981210; JP 2000524451 A 19981210; US 45856803 A 20030610; US 58110500 A 20000831