EP 1040201 A1 20001004 - SINGLE CHAIN MONOCLONAL ANTIBODY FUSION REAGENTS THAT REGULATE TRANSCRIPTION IN VIVO
Title (en)
SINGLE CHAIN MONOCLONAL ANTIBODY FUSION REAGENTS THAT REGULATE TRANSCRIPTION IN VIVO
Title (de)
EINKETTIGE MONOKLONAL-ANTIKÖRPER-FUSIONS-AGENTIEN DIE DIE IN VIVO TRANSKRIPTION REGULIEREN
Title (fr)
REACTIFS DE FUSION D'ANTICORPS MONOCLONAL A CHAINE UNIQUE REGULANT UNE TRANSCRIPTION IN VIVO
Publication
Application
Priority
US 9721407 W 19971128
Abstract (en)
[origin: WO9928502A1] A method of screening a DNA construct library for a single chain monoclonal antibody fusion reagent capable of binding a transcriptional associated biomolecule in vivo is described. Single chain monoclonal antibody fusion reagents capable of binding transcriptional associated biomolecules in vivo are provided. Single chain monoclonal antibody fusion reagents which are capable of regulating transcription in vivo are also provided. Therapeutic methods for regulating the transcription of a gene in vivo are also described. A method is further provided for screening a plurality of compounds for specific binding affinity with a single chain monoclonal antibody fusion reagent. A method is also described for diagnosing a physiological disorder manifested by an abnormal level of a transcription associated biomolecule. A DNA construct (pVP16Zeo) as well as primers for the construction and screening of single chain monoclonal antibody fusion reagent libraries to facilitate the isolation and production of single chain monoclonal antibody fusion reagents in yeast and E.coli are also provided. A kit for screening a DNA construct library for a single chain monoclonal antibody fusion reagent capable of binding a transcriptional associated biomolecule in vivo is also provided.
IPC 1-7
C12Q 1/68; C12Q 1/40; C07K 16/00; C12P 21/08; G01N 33/53; A61K 39/395; A61K 39/40; A61K 39/42; G01N 33/573
IPC 8 full level
C07K 16/18 (2006.01); C12N 15/10 (2006.01); C12P 21/08 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6897 (2018.01)
CPC (source: EP)
C07K 16/18 (2013.01); C12N 15/1055 (2013.01); C12Q 1/6897 (2013.01); A61K 2039/505 (2013.01); C07K 2317/622 (2013.01); Y02A 50/30 (2017.12)
Designated contracting state (EPC)
DE FR GB NL
DOCDB simple family (publication)
WO 9928502 A1 19990610; AU 3587599 A 19990616; EP 1040201 A1 20001004; EP 1040201 A4 20011121
DOCDB simple family (application)
US 9721407 W 19971128; AU 3587599 A 19971128; EP 97949553 A 19971128