EP 1042503 A1 20001011 - METHOD OF DETECTING MUTANT DNA BY MIPC AND PCR
Title (en)
METHOD OF DETECTING MUTANT DNA BY MIPC AND PCR
Title (de)
METHODE ZUR DETEKTION MUTANTER DNA DURCH MIPC UND PCR
Title (fr)
PROCEDE DE DETECTION D'ADN MUTANT PAR MIPC ET PCR
Publication
Application
Priority
- US 9823265 W 19981030
- US 6443797 P 19971031
- US 3906198 A 19980313
- US 5833798 A 19980410
- US 5858098 A 19980410
- US 12910598 A 19980804
- US 10331398 P 19981006
Abstract (en)
[origin: WO9923257A1] A method for detecting a putative mutant DNA in a sample of DNA includes the steps of amplifying the sample of DNA using PCR; hybridizing the amplified sample to form a mixture of homoduplexes and heteroduplexes; separating the mixture into fractions by Denaturing Matched Ion Polynucleotide Chromatography; and blind collecting the eluted fractions at a retention time corresponding to the retention time of the heteroduplex. The DNA in the blind collected fractions can be PCR amplified to obtain an increased amount of heteroduplex relative to homoduplex. The method is useful for determining the remission status of a patient in which the tissue-derived DNA sample contains a large background of wild type or where the putative mutant DNA is below the limit of detection.
IPC 1-7
C12Q 1/68; C12P 19/34; C07H 21/02; C07H 21/04; C07H 21/00; B01D 15/08; C02F 1/28
IPC 8 full level
B01D 15/08 (2006.01); C02F 1/28 (2006.01); C07H 21/00 (2006.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01); C12P 19/34 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP)
B01D 15/366 (2013.01); C12Q 1/6827 (2013.01)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9923257 A1 19990514; AU 1297599 A 19990524; EP 1042503 A1 20001011; EP 1042503 A4 20020116
DOCDB simple family (application)
US 9823265 W 19981030; AU 1297599 A 19981030; EP 98956451 A 19981030