EP 1042510 A1 20001011 - METHOD OF SELECTING FLANKING SEQUENCES WHICH CONVEY RELATIVE BINDING AFFINITIES TO A LIGAND BINDING SITE
Title (en)
METHOD OF SELECTING FLANKING SEQUENCES WHICH CONVEY RELATIVE BINDING AFFINITIES TO A LIGAND BINDING SITE
Title (de)
METHODE ZUR SELEKTION VON FLANKIERENDEN SEQUENZEN, WELCHE DIE BINDUNGSAFFINITÄTEN ZU EINER LIGANDENBINDESTELLE BEEINFLUSSEN
Title (fr)
PROCEDE DE SELECTION DE SEQUENCES ADJACENTES PORTEUSES D'AFFINITES DE LIAISON RELATIVES A UN SITE DE LIAISON AUX LIGANDS
Publication
Application
Priority
- US 9827461 W 19981223
- US 6861697 P 19971223
Abstract (en)
[origin: WO9932664A1] Methods of ranking the relative reactivity of nucleotide sequences flanking a ligand binding site. The invention involves, and is useful for, ranking populations of nucleic acid molecules, each containing a ligand binding site and flanking sequences on either side of the binding site. In an embodiment a method of ranking reactivities of polynucleotide flanking sequences is disclosed wherein the method includes (a) providing a plurality of different nucleic acid molecules, wherein each of the molecules has the same ligand binding site located adjacent to at least one flanking sequence; (b) exposing the molecules to a ligand selective for the binding site under conditions such that the relative binding affinity of the ligand for the binding site within at least two of the molecules is determined; and (c) ranking the relative binding affinities determined in step (b) to rank the relative reactivities of said polynucleotide flanking sequences. In another embodiment, a higher relative binding affinity for the ligand binding site within the molecule(s) corresponds to a higher relative reactivity of the flanking sequence(s).
IPC 1-7
IPC 8 full level
C12N 9/16 (2006.01); C12Q 1/44 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6811 (2018.01); G01N 33/50 (2006.01)
CPC (source: EP)
C12Q 1/6811 (2013.01)
Citation (search report)
See references of WO 9932664A1
Designated contracting state (EPC)
DE FR GB
DOCDB simple family (publication)
WO 9932664 A1 19990701; AU 2011499 A 19990712; EP 1042510 A1 20001011; JP 2001526905 A 20011225
DOCDB simple family (application)
US 9827461 W 19981223; AU 2011499 A 19981223; EP 98964895 A 19981223; JP 2000525581 A 19981223