Global patent index - EP 1042510 A1

EP 1042510 A1 2000-10-11 - METHOD OF SELECTING FLANKING SEQUENCES WHICH CONVEY RELATIVE BINDING AFFINITIES TO A LIGAND BINDING SITE

Title (en)

METHOD OF SELECTING FLANKING SEQUENCES WHICH CONVEY RELATIVE BINDING AFFINITIES TO A LIGAND BINDING SITE

Title (de)

METHODE ZUR SELEKTION VON FLANKIERENDEN SEQUENZEN, WELCHE DIE BINDUNGSAFFINITÄTEN ZU EINER LIGANDENBINDESTELLE BEEINFLUSSEN

Title (fr)

PROCEDE DE SELECTION DE SEQUENCES ADJACENTES PORTEUSES D'AFFINITES DE LIAISON RELATIVES A UN SITE DE LIAISON AUX LIGANDS

Publication

EP 1042510 A1 (EN)

Application

EP 98964895 A

Priority

  • US 9827461 W
  • US 6861697 P

Abstract (en)

[origin: WO9932664A1] Methods of ranking the relative reactivity of nucleotide sequences flanking a ligand binding site. The invention involves, and is useful for, ranking populations of nucleic acid molecules, each containing a ligand binding site and flanking sequences on either side of the binding site. In an embodiment a method of ranking reactivities of polynucleotide flanking sequences is disclosed wherein the method includes (a) providing a plurality of different nucleic acid molecules, wherein each of the molecules has the same ligand binding site located adjacent to at least one flanking sequence; (b) exposing the molecules to a ligand selective for the binding site under conditions such that the relative binding affinity of the ligand for the binding site within at least two of the molecules is determined; and (c) ranking the relative binding affinities determined in step (b) to rank the relative reactivities of said polynucleotide flanking sequences. In another embodiment, a higher relative binding affinity for the ligand binding site within the molecule(s) corresponds to a higher relative reactivity of the flanking sequence(s).

IPC 1-7 (main, further and additional classification)

C12Q 1/68; C07H 21/00; C12Q 1/44; G01N 33/50

IPC 8 full level (invention and additional information)

G01N 33/50 (2006.01); C12N 9/16 (2006.01); C12Q 1/44 (2006.01); C12Q 1/68 (2006.01)

CPC (invention and additional information)

C12Q 1/6811 (2013.01)

Citation (search report)

See references of WO 9932664A1

Designated contracting state (EPC)

DE FR GB

EPO simple patent family

WO 9932664 A1 19990701; AU 2011499 A 19990712; EP 1042510 A1 20001011; JP 2001526905 A 20011225

INPADOC legal status

2003-01-02 [18D] DEEMED TO BE WITHDRAWN

- Ref Legal Event Code: 18D

- Effective date: 20020702

2000-10-11 [17P] REQUEST FOR EXAMINATION FILED

- Ref Legal Event Code: 17P

- Effective date: 20000721

2000-10-11 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): DE FR GB