EP 1044378 A2 20001018 - REAL-TIME MONITORING OF AN ANALYTE BY CHROMATOGRAPHY USING AN ON-LINE ASSAY
Title (en)
REAL-TIME MONITORING OF AN ANALYTE BY CHROMATOGRAPHY USING AN ON-LINE ASSAY
Title (de)
ECHTZEITKONTROLLE EINES ANALYTEN DURCH CHROMATOGRAPHIE UNTER VERWENDUNG EINES ONLINE-ASSAYVERFAHRENS
Title (fr)
SUIVI EN TEMPS REEL D'UN ANALYTE PAR CHROMATOGRAPHIE EN LIGNE
Publication
Application
Priority
- US 9826172 W 19981209
- US 7015097 P 19971231
Abstract (en)
[origin: WO9934220A2] The presence of a contaminating analyte in a protein mixture may obscure detection of a protein of interest. In addition, antibody breakthrough is obscured by flow-through of contaminant proteins when loading cell culture fluid onto affinity columns. An on-line chromatographic assay described herein allows real-time measurement of contaminating analyte during purification or antibody breakthrough during loading and collection of a protein of interest in preparative chromatography. Breakthrough curves are generated allowing the user to end loading of cell culture fluid and/or to end effluent collection for maximization of efficient purification and preventing waste of cell culture fluid.
IPC 1-7
IPC 8 full level
G01N 30/62 (2006.01); G01N 30/02 (2006.01)
CPC (source: EP)
G01N 30/62 (2013.01); G01N 30/02 (2013.01); G01N 2333/65 (2013.01)
Citation (search report)
See references of WO 9934220A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9934220 A2 19990708; WO 9934220 A3 19990902; AU 2199099 A 19990719; EP 1044378 A2 20001018
DOCDB simple family (application)
US 9826172 W 19981209; AU 2199099 A 19981209; EP 98965982 A 19981209