Global Patent Index - EP 1045925 A1

EP 1045925 A1 2000-10-25 - ENZYME-SPECIFIC CLEAVABLE POLYNUCLEOTIDE SUBSTRATE AND ASSAY METHOD

Title (en)

ENZYME-SPECIFIC CLEAVABLE POLYNUCLEOTIDE SUBSTRATE AND ASSAY METHOD

Title (de)

ENZYMSPEZIFISCH SPALTBARES POLYNUKLEOTIDSUBSTRAT UND VERSUCHSVERFAHREN

Title (fr)

SUBSTRAT POLYNUCLEOTIDIQUE CLIVABLE SPECIFIQUE D'ENZYMES ET PROCEDE D'ANALYSE ASSOCIE

Publication

EP 1045925 A1 (EN)

Application

EP 98943281 A

Priority

  • US 9817311 W
  • US 526098 A

Abstract (en)

[origin: WO9935288A1] A reagent comprising an enzyme-specific cleavable polynucleotide (30, 31) substrate bearing quenched fluorescent moieties (32, 33) is provided, as is a method of making the same. The polynucleotide (30, 31) includes at least one fluorescent moiety (32) sufficiently close to another fluorescent moiety (33) to essentially quench fluorescence of the moieties (32, 33), wherein the fluorescent moieties (32, 33) become readily detectable by fluorometric techniques upon separation by cleaving the polynucleotide. A biological assay method is also provided wherein the reagent is combined with a test sample potentially containing the enzyme (36) being assayed wherein the enzyme (36) will cleave the polynucleotide to release the fluorescent moieties (32, 33), and produce an increase in fluorescence intensity. The assay method finds use in detection and identification of microorganisms, sterilization assurance, pharmaceutical discovery, enzyme assays, immunoassays, and other biological assays.

IPC 1-7 (main, further and additional classification)

C12Q 1/68

IPC 8 full level (invention and additional information)

G01N 33/50 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); G01N 21/75 (2006.01); G01N 33/533 (2006.01)

CPC (invention and additional information)

C12Q 1/6818 (2013.01)

Combination set (CPC)

C12Q 1/6818 + C12Q 2565/107 + C12Q 2565/1015 + C12Q 2521/301

Citation (search report)

See references of WO 9935288A1

Designated contracting state (EPC)

CH DE FR GB IT LI SE

EPO simple patent family

WO 9935288 A1 19990715; AU 9111098 A 19990726; CN 1285879 A 20010228; EP 1045925 A1 20001025; JP 2002508935 A 20020326

INPADOC legal status


2008-06-25 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20080209

2007-03-30 [REG HK WD] APPLICATIONS WITHDRAWN, DEEMED TO BE WITHDRAWN, OR REFUSED AFTER PUBLICATION IN HONG KONG

- Document: HK 1032984

2000-10-25 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20000710

2000-10-25 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): CH DE FR GB IT LI SE