EP 1045925 A1 20001025 - ENZYME-SPECIFIC CLEAVABLE POLYNUCLEOTIDE SUBSTRATE AND ASSAY METHOD
Title (en)
ENZYME-SPECIFIC CLEAVABLE POLYNUCLEOTIDE SUBSTRATE AND ASSAY METHOD
Title (de)
ENZYMSPEZIFISCH SPALTBARES POLYNUKLEOTIDSUBSTRAT UND VERSUCHSVERFAHREN
Title (fr)
SUBSTRAT POLYNUCLEOTIDIQUE CLIVABLE SPECIFIQUE D'ENZYMES ET PROCEDE D'ANALYSE ASSOCIE
Publication
Application
Priority
- US 9817311 W 19980820
- US 526098 A 19980109
Abstract (en)
[origin: WO9935288A1] A reagent comprising an enzyme-specific cleavable polynucleotide (30, 31) substrate bearing quenched fluorescent moieties (32, 33) is provided, as is a method of making the same. The polynucleotide (30, 31) includes at least one fluorescent moiety (32) sufficiently close to another fluorescent moiety (33) to essentially quench fluorescence of the moieties (32, 33), wherein the fluorescent moieties (32, 33) become readily detectable by fluorometric techniques upon separation by cleaving the polynucleotide. A biological assay method is also provided wherein the reagent is combined with a test sample potentially containing the enzyme (36) being assayed wherein the enzyme (36) will cleave the polynucleotide to release the fluorescent moieties (32, 33), and produce an increase in fluorescence intensity. The assay method finds use in detection and identification of microorganisms, sterilization assurance, pharmaceutical discovery, enzyme assays, immunoassays, and other biological assays.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6818 (2018.01); G01N 21/75 (2006.01); G01N 33/50 (2006.01); G01N 33/533 (2006.01)
CPC (source: EP KR)
C12Q 1/68 (2013.01 - KR); C12Q 1/6818 (2013.01 - EP)
Citation (search report)
See references of WO 9935288A1
Designated contracting state (EPC)
CH DE FR GB IT LI SE
DOCDB simple family (publication)
WO 9935288 A1 19990715; AU 9111098 A 19990726; CN 1285879 A 20010228; EP 1045925 A1 20001025; JP 2002508935 A 20020326; KR 100575407 B1 20060503; KR 20010033983 A 20010425
DOCDB simple family (application)
US 9817311 W 19980820; AU 9111098 A 19980820; CN 98813050 A 19980820; EP 98943281 A 19980820; JP 2000527669 A 19980820; KR 20007007574 A 20000708