Global Patent Index - EP 1047706 A2

EP 1047706 A2 20001102 - METHOD FOR CONDUCTING THE SYNTHESIS OF NUCLEIC ACID MOLECULES

Title (en)

METHOD FOR CONDUCTING THE SYNTHESIS OF NUCLEIC ACID MOLECULES

Title (de)

VERFAHREN ZUR SYNTHESE VON NUCLEINSÄUREMOLEKÜLEN

Title (fr)

PROCEDE PERMETTANT DE FAIRE LA SYNTHESE DE MOLECULES D'ACIDE NUCLEIQUE

Publication

EP 1047706 A2 20001102 (DE)

Application

EP 99919096 A 19990318

Priority

  • DE 9900755 W 19990318
  • DE 19812103 A 19980319

Abstract (en)

[origin: DE19812103A1] Synthesis of a nucleic acid (NA) comprises: (1) preparing a first NA (1) with at least one end that permits annealing and/or linkage of another NA (2); (2) annealing/linking (2) to the end of (1), with the other end of (2) masked; (3) masking the end of NA where (2) has annealed; (4) cleaving (2) at a predetermined site to remove the mask and to generate an end that can react (as above) with further NA; and (5) (v) repeating the cycle (ii)-(iv) at least once, using any suitable NA in step (ii). An Independent claim is also included for a kit comprising at least a ligase. a polymerase, a type II S-restriction enzyme and/or uracil-DNA-glycosylase, a pyrimidase and/or endonuclease III and a formamidinopyrimidine DNA glycosylase and/or a mismatch repair enzyme. The kit may also comprise: (1) a phosphatase, (2) a terminal transferase, and/or exonuclease; and (3) a synthetic matrix, optionally with starter NA attached to it.

IPC 1-7

C07H 21/00

IPC 8 full level

C12N 15/10 (2006.01); C12P 19/34 (2006.01)

CPC (source: EP)

C12N 15/10 (2013.01); C12N 15/66 (2013.01); C12P 19/34 (2013.01)

Citation (search report)

See references of WO 9947536A3

Designated contracting state (EPC)

AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

DE 19812103 A1 19990923; AU 3699899 A 19991011; EP 1047706 A2 20001102; WO 9947536 A2 19990923; WO 9947536 A3 20000127

DOCDB simple family (application)

DE 19812103 A 19980319; AU 3699899 A 19990318; DE 9900755 W 19990318; EP 99919096 A 19990318