EP 1047706 A2 20001102 - METHOD FOR CONDUCTING THE SYNTHESIS OF NUCLEIC ACID MOLECULES
Title (en)
METHOD FOR CONDUCTING THE SYNTHESIS OF NUCLEIC ACID MOLECULES
Title (de)
VERFAHREN ZUR SYNTHESE VON NUCLEINSÄUREMOLEKÜLEN
Title (fr)
PROCEDE PERMETTANT DE FAIRE LA SYNTHESE DE MOLECULES D'ACIDE NUCLEIQUE
Publication
Application
Priority
- DE 9900755 W 19990318
- DE 19812103 A 19980319
Abstract (en)
[origin: DE19812103A1] Synthesis of a nucleic acid (NA) comprises: (1) preparing a first NA (1) with at least one end that permits annealing and/or linkage of another NA (2); (2) annealing/linking (2) to the end of (1), with the other end of (2) masked; (3) masking the end of NA where (2) has annealed; (4) cleaving (2) at a predetermined site to remove the mask and to generate an end that can react (as above) with further NA; and (5) (v) repeating the cycle (ii)-(iv) at least once, using any suitable NA in step (ii). An Independent claim is also included for a kit comprising at least a ligase. a polymerase, a type II S-restriction enzyme and/or uracil-DNA-glycosylase, a pyrimidase and/or endonuclease III and a formamidinopyrimidine DNA glycosylase and/or a mismatch repair enzyme. The kit may also comprise: (1) a phosphatase, (2) a terminal transferase, and/or exonuclease; and (3) a synthetic matrix, optionally with starter NA attached to it.
IPC 1-7
IPC 8 full level
C12N 15/10 (2006.01); C12P 19/34 (2006.01)
CPC (source: EP)
C12N 15/10 (2013.01); C12N 15/66 (2013.01); C12P 19/34 (2013.01)
Citation (search report)
See references of WO 9947536A2
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
DE 19812103 A1 19990923; AU 3699899 A 19991011; EP 1047706 A2 20001102; WO 9947536 A2 19990923; WO 9947536 A3 20000127
DOCDB simple family (application)
DE 19812103 A 19980319; AU 3699899 A 19990318; DE 9900755 W 19990318; EP 99919096 A 19990318