Global Patent Index - EP 1047785 A1

EP 1047785 A1 2000-11-02 - MODIFIED DEACETOXYCEPHALOSPORIN C SYNTHASE (DAOCS) AND X-RAY STRUCTURE

Title (en)

MODIFIED DEACETOXYCEPHALOSPORIN C SYNTHASE (DAOCS) AND X-RAY STRUCTURE

Title (de)

MODIFIZIERTE DEACETOXYCEPHALOSPORIN C SYNTHASE (DAOCS) UND RÖNTGENSTRUKTUR

Title (fr)

DESACETOXYCEPHALOSPORINE C SYNTHASE (DAOCS) MODIFIES ET STRUCTURE AUX RAYONS X

Publication

EP 1047785 A1 (EN)

Application

EP 98962587 A

Priority

  • GB 9803860 W
  • GB 9727370 A
  • GB 9813644 A

Abstract (en)

[origin: WO9933994A1] Three-dimensional crystal structure(s) of deacetoxycephalosporin C synthase (DAOCS) are described. The X-ray co-ordinates provide precise 3-dimensional information of amino acids within the structure of DAOCS. Some of these are in complexes with iron and/or substrates. Information from the structures is used to modify enzymes of the cephalosporin biosynthesis pathway including DAOCS, deacetylcephalosporin C synthase DAOC/DACS, such that they accept unnatural substrates (e.g. penicillins G, V) in order to improve the production of beta-lactam antibiotics. The structures may be used to predict the structures of other 2-oxoglutarate dependent enzymes, thereby allowing the design of inhibitors, and new catalysts for the production of e.g. oxidised amino acids/peptides. Specific modifications of amino acid residues are proposed and exemplified.

IPC 1-7 (main, further and additional classification)

C12N 15/52; C12N 9/00; C12P 35/00

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); A61K 45/00 (2006.01); A61P 1/12 (2006.01); A61P 19/02 (2006.01); C07D 501/10 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 9/00 (2006.01); C12N 9/02 (2006.01); C12P 35/00 (2006.01)

CPC (invention and additional information)

C12N 9/0071 (2013.01)

Citation (search report)

See references of WO 9933994A1

Designated contracting state (EPC)

AT BE CH DE DK ES FI FR GB GR IE IT LI LU NL PT SE

EPO simple patent family

WO 9933994 A1 19990708; AU 1772099 A 19990719; CA 2316401 A1 19990708; CN 1284997 A 20010221; EP 1047785 A1 20001102; JP 2002500015 A 20020108; PL 341396 A1 20010409; SK 9762000 A3 20010212

INPADOC legal status


2006-09-06 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20060117

2004-06-09 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20040426

2000-11-02 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20000719

2000-11-02 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU NL PT SE