EP 1047791 A1 20001102 - USE OF A PROLINE-RICH SEQUENCE TO IMPROVE THE FUSOGENIC CHARACTER OF RETROVIRAL ENVELOPES
Title (en)
USE OF A PROLINE-RICH SEQUENCE TO IMPROVE THE FUSOGENIC CHARACTER OF RETROVIRAL ENVELOPES
Title (de)
VERWENDUNG VON EINER PROLINREICHEN SEQUENZ, UM DIE FUSOGENE EIGENSCHAFT VON RETROVIRALEN HÜLLPROTEINEN ZU ERHÖHEN
Title (fr)
UTILISATION D'UNE SEQUENCE RICHE EN PROLINE POUR AUGMENTER LE CARACTERE FUSOGENIQUE D'ENVELOPPES DE RETROVIRUS
Publication
Application
Priority
- FR 9900016 W 19990107
- FR 9800342 A 19980115
Abstract (en)
[origin: FR2773561A1] A proline-rich sequence corresponding to an envelope glycoprotein that has at least one mutation that prevents formation of at least one beta -turn in the polyproline helix, is new. A proline rich sequence has an amino acid sequence and secondary structure corresponding to that of a proline rich sequence of an envelope glycoprotein of an MLV amphotrope, retroviral MLV 10A1, MLV MCF, GALV, MLV xenotrope or FeLV. The sequence comprises at least one mutation, optionally in the C- or N-terminal region, such that it suppresses at least one beta -turn in the polyproline helix of the proline rich sequence. Independent claims are also included for the following: (1) a mutant protein corresponding to a glycoprotein envelope as above, in that a proline rich domain comprises: (a) at least one mutation such that at least one beta -turn in the polyproline helix is suppressed; or (b) is replaced with a proline rich domain of an MLV ecotropic retroviral glycoprotein envelope; and (2) a chimeric protein corresponding to a glycoprotein envelope as above, in that: (a) the proline-rich domain is replaced by the proline-rich domain of the envelope glycoprotein of a retroviral MLV ecotrope; or (b) the proline-rich domain comprises at least one mutation that suppresses a beta -turn (in the polyproline helix); and (c) at least one of the functional domains chosen from the linker domain, C domain or the transmembrane domain is replaced with a domain corresponding to the envelope of an ecotropic MLV.
IPC 1-7
IPC 8 full level
C07K 14/15 (2006.01); C07K 19/00 (2006.01); C12N 15/01 (2006.01); C12N 15/09 (2006.01); C12N 15/867 (2006.01)
CPC (source: EP)
C07K 14/005 (2013.01); C12N 15/86 (2013.01); C07K 2319/00 (2013.01); C12N 2740/13022 (2013.01); C12N 2740/13043 (2013.01); C12N 2740/13045 (2013.01); C12N 2810/6054 (2013.01)
Citation (search report)
See references of WO 9936561A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
FR 2773561 A1 19990716; CA 2317026 A1 19990722; EP 1047791 A1 20001102; JP 2002508975 A 20020326; WO 9936561 A1 19990722
DOCDB simple family (application)
FR 9800342 A 19980115; CA 2317026 A 19990107; EP 99900502 A 19990107; FR 9900016 W 19990107; JP 2000540262 A 19990107