EP 1047792 A1 20001102 - A METHOD FOR DEPOPULATING OF VERTEBRATE TESTIS AND FOR GENERATION OF TRANSGENIC SPECIES
Title (en)
A METHOD FOR DEPOPULATING OF VERTEBRATE TESTIS AND FOR GENERATION OF TRANSGENIC SPECIES
Title (de)
VERFAHREN ZUR ENTVÖLKERUNG VON VERTEBRATE-TESTIS UND ZUR GENERIERUNG VON TRANSGENEN TIERARTEN
Title (fr)
TECHNIQUE PERMETTANT DE DEPEUPLER UN TESTICULE DE VERTEBRE ET DE GENERER UNE ESPECE TRANSGENIQUE
Publication
Application
Priority
- US 9910573 W 19990513
- US 9824238 W 19981113
- US 19192098 A 19981113
- US 9908277 W 19990415
- US 29272399 A 19990415
Abstract (en)
[origin: WO0029601A1] A composition for in vitro and in vivo transfection of vertebrate male germ cells comprises a nucleic acid or transgene, and a gene delivery system, and optionally a protective internalizing agent, such as an endosomal lytic agent, a virus or a viral component, which is internalized by cells along with the transgene and which enhances gene transfer through the cytoplasm to the nucleus of the male germ cell. A method of genetically altering a vertebrate male germ cell in vivo employs a lentiviral-derived vector. A method of substantially depopulating a vertebrate testis employs a combination of a dose of an alkylating agent, such as busulfan, chlorambucil, cyclophosphamide, melphalan, or ethyl ethanesulfonic acid, and a dose of gamma radiation. A pharmaceutical preparation and a transfer kit utilize the composition. A method for introducing a polynucleotide into vertebrate male germ cells comprises the administration of the composition to a vertebrate. A method for isolating or selecting transfected cells utilizes a reporter gene, and a method for administering transfected male germ cells utilizes male germ cells which have been transfected in vitro.
IPC 1-7
C12N 15/89; C12N 15/86; C12N 15/88; C12N 5/06; A01K 67/027; A61K 48/00
IPC 8 full level
A01K 67/027 (2006.01); A61K 9/127 (2006.01); A61K 31/192 (2006.01); A61K 31/255 (2006.01); A61K 31/573 (2006.01); A61K 31/675 (2006.01); A61K 35/52 (2006.01); A61K 35/76 (2006.01); A61K 38/00 (2006.01); A61K 47/42 (2006.01); A61K 48/00 (2006.01); A61L 27/00 (2006.01); A61P 1/00 (2006.01); A61P 3/10 (2006.01); A61P 9/10 (2006.01); A61P 9/12 (2006.01); A61P 15/08 (2006.01); A61P 25/14 (2006.01); A61P 25/18 (2006.01); A61P 25/24 (2006.01); A61P 43/00 (2006.01); C12N 5/076 (2010.01); C12N 5/10 (2006.01); C12N 15/09 (2006.01); A61K 35/12 (2015.01)
CPC (source: EP)
A01K 67/027 (2013.01); A01K 67/0275 (2013.01); A61K 48/00 (2013.01); A61P 1/00 (2017.12); A61P 3/10 (2017.12); A61P 9/10 (2017.12); A61P 9/12 (2017.12); A61P 15/08 (2017.12); A61P 25/14 (2017.12); A61P 25/18 (2017.12); A61P 25/24 (2017.12); A61P 43/00 (2017.12); C12N 5/061 (2013.01); A01K 2217/05 (2013.01); A61K 35/12 (2013.01); C12N 2510/00 (2013.01); C12N 2510/02 (2013.01); C12N 2799/021 (2013.01); C12N 2799/022 (2013.01); C12N 2799/027 (2013.01)
Citation (search report)
See references of WO 0029601A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0029601 A1 20000525; AU 4077199 A 20000605; EP 1047792 A1 20001102; JP 2003525581 A 20030902
DOCDB simple family (application)
US 9910573 W 19990513; AU 4077199 A 19990513; EP 99924219 A 19990513; JP 2000582582 A 19990513