EP 1047794 A2 20001102 - METHOD FOR THE DETECTION OR NUCLEIC ACID OF NUCLEIC ACID SEQUENCES
Title (en)
METHOD FOR THE DETECTION OR NUCLEIC ACID OF NUCLEIC ACID SEQUENCES
Title (de)
VERFAHREN ZUM NACHWEIS VON NUKLEINSÄURESEQUENZEN
Title (fr)
PROCEDE DE DETECTION DE SEQUENCES NUCLEOTIDIQUES
Publication
Application
Priority
- EP 99907365 A 19990113
- EP 9900161 W 19990113
- EP 98100485 A 19980113
Abstract (en)
[origin: WO9936571A2] The present invention relates to a novel method for the detection of a nucleic acid sequence within a nucleic acid molecule. The method of the invention relies on the combination of nucleic acid protection, ligation of oligonucleotides to the protected nucleic acid molecules and amplification of the ligation products. The detection of the amplified products is advantageously effected by converting the same to the single-stranded form and hybridizing the single-stranded form thereof to an array of single-stranded nucleic acid molecules of at least partially predetermined sequence fixed to a solid support. The solid support is preferably a chip. Detection of hybridized molecules can be effected according to conventional methods. The present invention additionally relates to a kit for carrying out the method of the invention.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6837 (2018.01); C12Q 1/6855 (2018.01); G01N 30/88 (2006.01)
CPC (source: EP)
C12Q 1/6837 (2013.01); C12Q 1/6855 (2013.01)
Citation (search report)
See references of WO 9936571A2
Designated contracting state (EPC)
AT CH DE FR GB LI
DOCDB simple family (publication)
WO 9936571 A2 19990722; WO 9936571 A3 19991021; CA 2318371 A1 19990722; EP 1047794 A2 20001102; JP 2002517981 A 20020625
DOCDB simple family (application)
EP 9900161 W 19990113; CA 2318371 A 19990113; EP 99907365 A 19990113; JP 2000540272 A 19990113