Global Patent Index - EP 1049766 A1

EP 1049766 A1 2000-11-08 - METHOD AND DEVICE FOR CELL LYSIS

Title (en)

METHOD AND DEVICE FOR CELL LYSIS

Title (de)

VERFAHREN UND ANLAGE FÜR ZELLYSE

Title (fr)

PROCEDE ET DISPOSITIF DE LYSE CELLULAIRE

Publication

EP 1049766 A1 (FR)

Application

EP 99900963 A

Priority

  • FR 9900105 W
  • FR 9800815 A

Abstract (en)

[origin: FR2773818A1] Continuous method of lysis of bacteria comprises mixing bacterial liquid and a lysis reagent and immediately pouring the mixture into a pipe. The pipe has a constant flux, where the flow of the flux is adapted, as a function of the diameter and the length of the pipe, to give a homogeneous bacterial lysate upon exiting the pipe. Independent claims are also included for: (1) a method of extracting and purifying nucleic acids, especially plasmids, from a bacterial suspension, by a lysis method as above, followed by neutralization at a second determined point in the process to precipitate or flocculate undesirable cellular components, the duration being determined by the distance separating the lysis and neutralization and by the rate of flux over this distance; (2) a device for carrying out the above processes comprising a pipe (3) for forming a flow of bacterial suspension, a pipe (5) for forming a flow of lysing agent from an alkaline source of lysing agent such as a reservoir (4), these two pipes merging at a point (6). There is also a third small diameter pipe (7) of predetermined length which extends from the aforementioned point, as well as means such as a pump (13) to create a flow in the pipes, the length, the diameter and the flow of the third pipe being such that they give a homogeneous mixture leading to the formation of a homogeneous lysate.

IPC 1-7 (main, further and additional classification)

C12N 1/06

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); C12M 1/00 (2006.01); C12N 1/06 (2006.01)

CPC (invention and additional information)

C12N 1/06 (2013.01)

Citation (search report)

See references of WO 9937750A1

Designated contracting state (EPC)

AT BE CH DE DK ES FI FR GB GR IE IT LI LU NL PT SE

EPO simple patent family

FR 2773818 A1 19990723; FR 2773818 B1 20000218; AU 2060499 A 19990809; AU 756179 B2 20030109; CA 2319021 A1 19990729; EP 1049766 A1 20001108; JP 2002500878 A 20020115; NZ 505865 A 20021220; WO 9937750 A1 19990729

INPADOC legal status


2005-03-09 [18W] WITHDRAWN

- Effective date: 20050110

2003-09-03 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20030722

2000-11-08 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20000821

2000-11-08 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU NL PT SE