Global Patent Index - EP 1050581 A1

EP 1050581 A1 2000-11-08 - METHOD FOR DETECTING ACETYLTRANSFERASE AND DEACETYLASE ACTIVITIES AND METHOD FOR SCREENING INHIBITORS OR ACCELERATORS OF THESE ENZYMES

Title (en)

METHOD FOR DETECTING ACETYLTRANSFERASE AND DEACETYLASE ACTIVITIES AND METHOD FOR SCREENING INHIBITORS OR ACCELERATORS OF THESE ENZYMES

Title (de)

VERFAHREN ZUR DETEKTION VON ACETYLTRANSFERASE- UND DEACETYLASEAKTIVITÄTEN UND VERFAHREN ZUM AUFFINDEN VON HEMMSTOFFEN ODER BESCHLEUNIGERN DIESER ENZYME

Title (fr)

PROCEDE POUR DETECTER DES ACTIVITES ACETYLTRANSFERASE ET DESACETYLASE, AINSI QUE PROCEDE POUR CRIBLER DES INHIBITEURS OU DES ACCELERATEURS DE CES ENZYMES

Publication

EP 1050581 A1 (EN)

Application

EP 99901120 A

Priority

  • JP 9900191 W
  • JP 917198 A

Abstract (en)

A method for simply and conveniently detecting acetyltransferase and deacetylase activities of proteins by executing an acetylation reaction of a peptide substrate with an acetyltransferase, or a deacetylation reaction of an acetylated peptide substrate with a deacetylase, and after the completion of these reactions, detecting the acetyl group bound to the peptide substrate by using an anti-acetylated peptide antibody. This system for detecting acetyltransferase and deacetylase activities using the anti-acetylated peptide antibody enables screening inhibitors or enhancers of acetyltransferase and deacetylase. A system for screening deacetylase inhibitors or acetyltransferase enhancers using cultured cells is also provided.

IPC 1-7 (main, further and additional classification)

C12N 15/12; C12N 9/10; C12P 21/08; C12Q 1/48; C12Q 1/68; G01N 33/563

IPC 8 full level (invention and additional information)

C07K 16/18 (2006.01); C07K 16/40 (2006.01); C12N 15/12 (2006.01); C12P 21/08 (2006.01); C12Q 1/48 (2006.01); G01N 33/573 (2006.01)

CPC (invention and additional information)

C07K 5/12 (2013.01); C07K 16/18 (2013.01); C07K 16/40 (2013.01); C12Q 1/48 (2013.01); G01N 2333/91051 (2013.01); G01N 2500/00 (2013.01)

Designated contracting state (EPC)

CH DE FR GB IT LI

EPO simple patent family

WO 9936532 A1 19990722; EP 1050581 A1 20001108; EP 1050581 A4 20051019

INPADOC legal status


2010-11-17 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20100526

2007-05-02 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20060317

2005-10-19 [A4] SUPPLEMENTARY SEARCH REPORT

- Effective date: 20050905

2005-10-19 [RIC1] CLASSIFICATION (CORRECTION)

- IPC: 7C 12Q 1/48 A

2005-10-19 [RIC1] CLASSIFICATION (CORRECTION)

- IPC: 7G 01N 33/573 B

2001-04-04 [RIN1] INVENTOR (CORRECTION)

- Inventor name: TAYA, YOICHI BIOLOGY DIVISION

2001-04-04 [RIN1] INVENTOR (CORRECTION)

- Inventor name: TAMAI, KATSUYUKI

2001-04-04 [RIN1] INVENTOR (CORRECTION)

- Inventor name: MIYAZAKI, TOSHIAKI

2000-11-08 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20000821

2000-11-08 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): CH DE FR GB IT LI