Title (en)

METHOD FOR DETECTING ACETYLTRANSFERASE AND DEACETYLASE ACTIVITIES AND METHOD FOR SCREENING INHIBITORS OR ACCELERATORS OF THESE ENZYMES

Title (de)

VERFAHREN ZUR DETEKTION VON ACETYLTRANSFERASE- UND DEACETYLASEAKTIVITÄTEN UND VERFAHREN ZUM AUFFINDEN VON HEMMSTOFFEN ODER BESCHLEUNIGERN DIESER ENZYME

Title (fr)

PROCEDE POUR DETECTER DES ACTIVITES ACETYLTRANSFERASE ET DESACETYLASE, AINSI QUE PROCEDE POUR CRIBLER DES INHIBITEURS OU DES ACCELERATEURS DE CES ENZYMES

Publication

EP 1050581 A1 20001108 (EN)

Application

EP 99901120 A 19990120

Priority

• JP 9900191 W 19990120
• JP 917198 A 19980120

Abstract (en)

A method for simply and conveniently detecting acetyltransferase and deacetylase activities of proteins by executing an acetylation reaction of a peptide substrate with an acetyltransferase, or a deacetylation reaction of an acetylated peptide substrate with a deacetylase, and after the completion of these reactions, detecting the acetyl group bound to the peptide substrate by using an anti-acetylated peptide antibody. This system for detecting acetyltransferase and deacetylase activities using the anti-acetylated peptide antibody enables screening inhibitors or enhancers of acetyltransferase and deacetylase. A system for screening deacetylase inhibitors or acetyltransferase enhancers using cultured cells is also provided.

IPC 1-7

C12N 15/12; C12P 21/08; C12Q 1/68; C12N 9/10; C12Q 1/48; G01N 33/563

IPC 8 full level

C07K 16/18 (2006.01); C07K 16/40 (2006.01); C12N 15/12 (2006.01); C12P 21/08 (2006.01); C12Q 1/48 (2006.01); G01N 33/573 (2006.01)

CPC (source: EP)

C07K 5/12 (2013.01); C07K 16/18 (2013.01); C07K 16/40 (2013.01); C12Q 1/48 (2013.01); G01N 2333/91051 (2013.01); G01N 2500/00 (2013.01)

Designated contracting state (EPC)

CH DE FR GB IT LI

DOCDB simple family (publication)

WO 9936532 A1 19990722; EP 1050581 A1 20001108; EP 1050581 A4 20051019

DOCDB simple family (application)

JP 9900191 W 19990120; EP 99901120 A 19990120