EP 1053358 A1 20001122 - THE USE OF HIV-1 INTEGRASE IN SCREENING HIV-1 DRUG CANDIDATES
Title (en)
THE USE OF HIV-1 INTEGRASE IN SCREENING HIV-1 DRUG CANDIDATES
Title (de)
VERWENDUNG VON HIV-1 INTEGRASE BEIM SCREENING VON POTENTIELLEN HIV-1 ARZNEISTOFFEN
Title (fr)
UTILISATION DE L'INTEGRASE DU VIH-1 DANS LE CRIBLAGE DE MEDICAMENTS CANDIDATS DIRIGES CONTRE LE VIH-1
Publication
Application
Priority
- US 9902912 W 19990210
- US 7427498 P 19980210
Abstract (en)
[origin: WO9940227A1] The present invention harnesses the roles of IN to screen drug candidates to inhibit HIV-1. The present invention uses IN mutants and fusion proteins to assess the effectiveness of a drug candidate in inhibiting a native IN function. The retrovirus integrase protein (IN) is essential for integration of the viral DNA into host cell chromosomes as well as for retroviruses in general. The ability of a drug candidate to inhibit retroviral replication utilizes a monitoring system such as a host cell culture infected with a retrovirus. After exposing the monitoring system to the drug candidate, the retroviral life cycle within the monitoring system is monitored and compared to monitoring system life cycle function of integrase mutant retrovirus. Alternatively, an integrase fusion protein of either vpr or vpx is added to a monitoring system infected with a retrovirus expressing wild-type integrase following exposure to a drug candidate. Changes in retrovirus life cycle function are monitored for suppression of a wild-type integrase function indicating retroviral replication has been comprised.
IPC 1-7
IPC 8 full level
C07K 14/16 (2006.01)
CPC (source: EP)
C07K 14/005 (2013.01); C07K 2319/00 (2013.01); C12N 2740/16222 (2013.01); C12N 2740/16322 (2013.01)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9940227 A1 19990812; AU 2597599 A 19990823; EP 1053358 A1 20001122; EP 1053358 A4 20020814
DOCDB simple family (application)
US 9902912 W 19990210; AU 2597599 A 19990210; EP 99905936 A 19990210