Global patent index - EP 1054977 A1

EP 1054977 A1 2000-11-29 - METHOD FOR PRODUCING BIOTIN

Title (en)

METHOD FOR PRODUCING BIOTIN

Title (de)

VERFAHREN ZUR HERSTELLUNG VON BIOTIN

Title (fr)

PROCEDE POUR LA PREPARATION DE BIOTINE

Publication

EP 1054977 A1 (DE)

Application

EP 99908923 A

Priority

  • DE 19806872 A
  • EP 9901052 W

Abstract (en)

[origin: DE19806872A1] Preparation of biotin (I) comprises expressing, in a prokaryotic or eukaryotic host capable of producing (I): (a) an S-adenosyl-methionine synthase sequence (1), of 1155 bp (reproduced); and (b) at least one of the other biotin biosynthesis genes bioS1, 2 or 3 (sequences of 1206 (3), 1215 (5) and 1221 (7) bp, respectively, reproduced). Preparation of biotin (I) comprises expressing, in a prokaryotic or eukaryotic host capable of producing (I): (a) an S-adenosyl-methionine synthase sequence (1), of 1155 bp (reproduced); and (b) at least one of the other biotin biosynthesis genes bioS1, 2 or 3 (sequences of 1206 (3), 1215 (5) and 1221 (7) bp, respectively, reproduced). The transformed cells are cultured and synthesized (I) used directly, after removal of biomass, or after purification. Independent claims are also included for the following: (1) a gene construct containing (1) and at least one of (3), (5) or (7), or their functional variants, analogs or derivatives, linked to either one or more regulatory signals for increased gene and/or protein expression, and/or to their native regulators; (2) organisms containing the construct of (a); and (3) use of the bioS3 gene (7), or its variants, analogs and derivatives, alone or in combination with other biotin synthesis genes, for production of (I).

IPC 1-7 (main, further and additional classification)

C12N 15/54; C12P 17/18; C12N 9/10; C12N 1/21

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 9/10 (2006.01); C12N 15/54 (2006.01); C12N 15/60 (2006.01); C12P 17/18 (2006.01); C12R 1/19 (2006.01)

CPC (invention and additional information)

C12N 9/1085 (2013.01); C12N 9/13 (2013.01); C12P 17/186 (2013.01)

Designated contracting state (EPC)

AT CH DE ES FI FR GB IT LI NL

EPO simple patent family

DE 19806872 A1 19990826; CA 2321264 A1 19990826; CN 1210403 C 20050713; CN 1291232 A 20010411; EP 1054977 A1 20001129; IL 137310 D0 20010724; JP 2002504338 A 20020212; WO 9942591 A1 19990826

INPADOC legal status

2006-05-17 [18D] DEEMED TO BE WITHDRAWN

- Ref Legal Event Code: 18D

- Effective date: 20051027

2004-08-11 [17Q] FIRST EXAMINATION REPORT

- Ref Legal Event Code: 17Q

- Effective date: 20040624

2000-11-29 [17P] REQUEST FOR EXAMINATION FILED

- Ref Legal Event Code: 17P

- Effective date: 20000623

2000-11-29 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): AT CH DE ES FI FR GB IT LI NL