EP 1057036 A1 20001206 - FLOW CYTOMETRIC DETECTION OF CONFORMATIONS OF pRB IN SINGLE CELLS
Title (en)
FLOW CYTOMETRIC DETECTION OF CONFORMATIONS OF pRB IN SINGLE CELLS
Title (de)
DURCHFLUSSCYTOMETRISCHER NACHWEIS VON KONFORMATIONEN VON PRB IN EINZELZELLEN
Title (fr)
DETECTION PAR CYTOMETRIE DE FLUX DE CONFORMATIONS DE pRB DANS DES CELLULES INDIVIDUELLES
Publication
Application
Priority
- US 9904015 W 19990224
- US 7590898 P 19980225
Abstract (en)
[origin: WO9944067A1] Methods, reagents, and kits are provided that permit flow cytometric determination of the phosphorylation status of retinoblastoma susceptibility gene protein (pRB) in individual cells. Methods are described that permit the hypophosphorylated, active, form of pRB to be measured either as an absolute quantity or as a proportion of total cellular pRB. Further described are methods that permit pRB phosphorylation status to be correlated with cell cycle phase and with protein components of the cell cycle. Screening of chemical compounds for antiproliferative and antineoplastic activity using the flow cytometric assays is demonstrated. Reagent kits that facilitate the subject methods are also provided.
IPC 1-7
IPC 8 full level
C07K 16/00 (2006.01); C07K 16/40 (2006.01); G01N 33/15 (2006.01); G01N 33/50 (2006.01); G01N 33/53 (2006.01); G01N 33/543 (2006.01); G01N 33/68 (2006.01)
CPC (source: EP)
G01N 33/5011 (2013.01); G01N 33/53 (2013.01); G01N 33/543 (2013.01); G01N 33/68 (2013.01); G01N 33/6842 (2013.01); G01N 2333/4736 (2013.01); G01N 2333/9121 (2013.01)
Citation (search report)
See references of WO 9944067A1
Designated contracting state (EPC)
DE FR GB IT
DOCDB simple family (publication)
WO 9944067 A1 19990902; AU 3310099 A 19990915; EP 1057036 A1 20001206; JP 2002505433 A 20020219
DOCDB simple family (application)
US 9904015 W 19990224; AU 3310099 A 19990224; EP 99936123 A 19990224; JP 2000533764 A 19990224