Global Patent Index - EP 1057036 A1

EP 1057036 A1 2000-12-06 - FLOW CYTOMETRIC DETECTION OF CONFORMATIONS OF pRB IN SINGLE CELLS

Title (en)

FLOW CYTOMETRIC DETECTION OF CONFORMATIONS OF pRB IN SINGLE CELLS

Title (de)

DURCHFLUSSCYTOMETRISCHER NACHWEIS VON KONFORMATIONEN VON PRB IN EINZELZELLEN

Title (fr)

DETECTION PAR CYTOMETRIE DE FLUX DE CONFORMATIONS DE pRB DANS DES CELLULES INDIVIDUELLES

Publication

EP 1057036 A1 (EN)

Application

EP 99936123 A

Priority

  • US 9904015 W
  • US 7590898 P

Abstract (en)

[origin: WO9944067A1] Methods, reagents, and kits are provided that permit flow cytometric determination of the phosphorylation status of retinoblastoma susceptibility gene protein (pRB) in individual cells. Methods are described that permit the hypophosphorylated, active, form of pRB to be measured either as an absolute quantity or as a proportion of total cellular pRB. Further described are methods that permit pRB phosphorylation status to be correlated with cell cycle phase and with protein components of the cell cycle. Screening of chemical compounds for antiproliferative and antineoplastic activity using the flow cytometric assays is demonstrated. Reagent kits that facilitate the subject methods are also provided.

IPC 1-7 (main, further and additional classification)

G01N 33/68; G01N 33/53; G01N 33/543

IPC 8 full level (invention and additional information)

C07K 16/00 (2006.01); C07K 16/40 (2006.01); G01N 33/15 (2006.01); G01N 33/50 (2006.01); G01N 33/53 (2006.01); G01N 33/543 (2006.01); G01N 33/68 (2006.01)

CPC (invention and additional information)

G01N 33/6842 (2013.01); G01N 33/5011 (2013.01); G01N 33/53 (2013.01); G01N 33/543 (2013.01); G01N 33/68 (2013.01); G01N 2333/4736 (2013.01); G01N 2333/9121 (2013.01)

Citation (search report)

See references of WO 9944067A1

Designated contracting state (EPC)

DE FR GB IT

EPO simple patent family

WO 9944067 A1 19990902; AU 3310099 A 19990915; EP 1057036 A1 20001206; JP 2002505433 A 20020219

INPADOC legal status


2009-10-07 [18W] WITHDRAWN

- Effective date: 20090828

2007-05-23 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20070419

2000-12-06 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20000816

2000-12-06 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): DE FR GB IT