EP 1060246 A2 20001220 - METHOD FOR SELECTING RIBOZYMES WHICH ARE CAPABLE OF COVALENTLY MODIFYING THE RIBONUCLEIC ACIDS ON 2'-OH-GROUPS IN TRANS
Title (en)
METHOD FOR SELECTING RIBOZYMES WHICH ARE CAPABLE OF COVALENTLY MODIFYING THE RIBONUCLEIC ACIDS ON 2'-OH-GROUPS IN TRANS
Title (de)
VERFAHREN ZUR SELEKTION VON RIBOZYMEN, DIE RIBONUCLEINSÄUREN IN TRANS AN 2' -OH-GRUPPEN KOVALENT MODIFIZIEREN KÖNNEN
Title (fr)
PROCEDE DE SELECTION DE RIBOZYMES CAPABLES DE MODIFIER PAR COVALENCE LES ACIDES RIBONUCLEIQUES, EN TRANS, SUR LES GROUPES 2'-OH
Publication
Application
Priority
- DE 19801153 A 19980114
- EP 9900181 W 19990114
Abstract (en)
[origin: DE19801153A1] A method to select a ribozyme that can covalently modify the 2'-OH group of ribonucleic acids in trans is new. A method to select a ribozyme that can covalently modify the 2'-OH group of ribonucleic acids in trans, comprises: incubating a ribonucleic acid library with a reactant that can react with a ribonucleic acid 2'-OH group; selection and isolation of the ribonucleic acid that covalently binds to the reactant; amplifying the ribonucleic acid of step (b) to form cDNA; determining the sequence of the selected ribonucleic acid; cleavage of the selected ribonucleic acid in a substrate part (I) by a catalytic part (II = ribozyme); and testing whether the catalytically active part (II) can covalently modify the substrate part (I). Independent claims are also included for: a ribozyme obtained by a method as above; a DNA sequence that encodes a ribozyme as in (1); a vector containing DNA as in (2); a host cell containing a vector as in (3); and a method to produce a ribozyme as in (1).
IPC 1-7
IPC 8 full level
C12N 15/11 (2006.01); C12N 15/113 (2010.01); A61K 38/00 (2006.01)
CPC (source: EP)
C12N 15/113 (2013.01); A61K 38/00 (2013.01); C12N 2310/12 (2013.01); C12N 2799/027 (2013.01)
Citation (search report)
See references of WO 9936517A2
Designated contracting state (EPC)
DE FR GB IT NL
DOCDB simple family (publication)
DE 19801153 A1 19990715; EP 1060246 A2 20001220; WO 9936517 A2 19990722; WO 9936517 A3 19990923
DOCDB simple family (application)
DE 19801153 A 19980114; EP 9900181 W 19990114; EP 99907369 A 19990114