Global Patent Index - EP 1060246 A2

EP 1060246 A2 2000-12-20 - METHOD FOR SELECTING RIBOZYMES WHICH ARE CAPABLE OF COVALENTLY MODIFYING THE RIBONUCLEIC ACIDS ON 2'-OH-GROUPS IN TRANS

Title (en)

METHOD FOR SELECTING RIBOZYMES WHICH ARE CAPABLE OF COVALENTLY MODIFYING THE RIBONUCLEIC ACIDS ON 2'-OH-GROUPS IN TRANS

Title (de)

VERFAHREN ZUR SELEKTION VON RIBOZYMEN, DIE RIBONUCLEINSÄUREN IN TRANS AN 2' -OH-GRUPPEN KOVALENT MODIFIZIEREN KÖNNEN

Title (fr)

PROCEDE DE SELECTION DE RIBOZYMES CAPABLES DE MODIFIER PAR COVALENCE LES ACIDES RIBONUCLEIQUES, EN TRANS, SUR LES GROUPES 2'-OH

Publication

EP 1060246 A2 (DE)

Application

EP 99907369 A

Priority

  • DE 19801153 A
  • EP 9900181 W

Abstract (en)

[origin: DE19801153A1] A method to select a ribozyme that can covalently modify the 2'-OH group of ribonucleic acids in trans is new. A method to select a ribozyme that can covalently modify the 2'-OH group of ribonucleic acids in trans, comprises: incubating a ribonucleic acid library with a reactant that can react with a ribonucleic acid 2'-OH group; selection and isolation of the ribonucleic acid that covalently binds to the reactant; amplifying the ribonucleic acid of step (b) to form cDNA; determining the sequence of the selected ribonucleic acid; cleavage of the selected ribonucleic acid in a substrate part (I) by a catalytic part (II = ribozyme); and testing whether the catalytically active part (II) can covalently modify the substrate part (I). Independent claims are also included for: a ribozyme obtained by a method as above; a DNA sequence that encodes a ribozyme as in (1); a vector containing DNA as in (2); a host cell containing a vector as in (3); and a method to produce a ribozyme as in (1).

IPC 1-7 (main, further and additional classification)

C12N 15/11; A61K 31/70; C12N 9/00; C12N 15/86; C12Q 1/68

IPC 8 full level (invention and additional information)

C12N 15/113 (2010.01); A61K 38/00 (2006.01)

CPC (invention and additional information)

C12N 15/113 (2013.01); A61K 38/00 (2013.01); C12N 2310/12 (2013.01); C12N 2799/027 (2013.01)

Citation (search report)

See references of WO 9936517A3

Designated contracting state (EPC)

DE FR GB IT NL

EPO simple patent family

DE 19801153 A1 19990715; EP 1060246 A2 20001220; WO 9936517 A2 19990722; WO 9936517 A3 19990923

INPADOC legal status


2004-12-29 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20040629

2004-06-02 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20040416

2000-12-20 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20001017

2000-12-20 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): DE FR GB IT NL