EP 1060266 A1 20001220 - METHOD FOR IDENTIFYING MISMATCH REPAIR GLYCOSYLASE REACTIVE SITES, COMPOUND AND USES THEREOF
Title (en)
METHOD FOR IDENTIFYING MISMATCH REPAIR GLYCOSYLASE REACTIVE SITES, COMPOUND AND USES THEREOF
Title (de)
VEFRAHREN ZUR IDENTIFIZIERUNG REAKTIVER SITES FÜR GLYKOSYLASE ZUR REPARATUR VON FEHLBASENPAARUNGEN, WIRKSTOFF, SOWIE VERWENDUNGEN DERSELBEN
Title (fr)
PROCEDE PERMETTANT D'IDENTIFIER LES SITES REACTIFS DES GLYCOSYLASES DE REPARATION DES MESAPPARIEMENTS, COMPOSE ET UTILISATIONS DE CE DERNIER
Publication
Application
Priority
- US 9903821 W 19990223
- US 7554298 P 19980223
- US 22422798 A 19981230
Abstract (en)
[origin: WO9942622A1] The present application discloses a method of identifying mutations in a target DNA sequence. The method involves: (a) hybridizing the target DNA sequence with a control DNA sequence wherein said control DNA sequence is the wild-type DNA sequence corresponding to the target DNA sequence to create a duplex; (b) treating the duplex to remove any spontaneous aldehydes; (c) reacting the duplex with a repair glycosylase to convert any mismatched sites in the duplex to reactive sites containing an aldehyde-containing abasic site; (d) reacting the duplex with a compound of the formula X-Z-Y, wherein X is a detectable moiety, Y is NHNH2, O-NH2 or NH2, and Z is a hydrocarbon, alkylhydroxy, alkylethoxy, alkylester, alkylether, alkylamide or alkylamine, wherein Z may be substituted or unsubstituted; and wherein Z may contain a cleavable group; for a sufficient time and under conditions to covalently bind to the reactive sites; (e) detecting the bound compound to identify sites of mismatches; (f) determining where the mismatch occurs; and (g) determining whether the mismatch is a mutation or polymorphisms.
IPC 1-7
IPC 8 full level
C07D 311/16 (2006.01); C07D 311/82 (2006.01); C07D 495/04 (2006.01); C12Q 1/68 (2006.01); G01N 33/53 (2006.01); G01N 37/00 (2006.01)
CPC (source: EP)
C12Q 1/6827 (2013.01); C12Q 1/6837 (2013.01)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9942622 A1 19990826; AU 2780799 A 19990906; AU 756384 B2 20030109; CA 2321119 A1 19990826; EP 1060266 A1 20001220; EP 1060266 A4 20040922; JP 2002504352 A 20020212
DOCDB simple family (application)
US 9903821 W 19990223; AU 2780799 A 19990223; CA 2321119 A 19990223; EP 99908354 A 19990223; JP 2000532560 A 19990223