Global Patent Index - EP 1060266 A1

EP 1060266 A1 2000-12-20 - METHOD FOR IDENTIFYING MISMATCH REPAIR GLYCOSYLASE REACTIVE SITES, COMPOUND AND USES THEREOF

Title (en)

METHOD FOR IDENTIFYING MISMATCH REPAIR GLYCOSYLASE REACTIVE SITES, COMPOUND AND USES THEREOF

Title (de)

VEFRAHREN ZUR IDENTIFIZIERUNG REAKTIVER SITES FÜR GLYKOSYLASE ZUR REPARATUR VON FEHLBASENPAARUNGEN, WIRKSTOFF, SOWIE VERWENDUNGEN DERSELBEN

Title (fr)

PROCEDE PERMETTANT D'IDENTIFIER LES SITES REACTIFS DES GLYCOSYLASES DE REPARATION DES MESAPPARIEMENTS, COMPOSE ET UTILISATIONS DE CE DERNIER

Publication

EP 1060266 A1 (EN)

Application

EP 99908354 A

Priority

  • US 9903821 W
  • US 7554298 P
  • US 22422798 A

Abstract (en)

[origin: WO9942622A1] The present application discloses a method of identifying mutations in a target DNA sequence. The method involves: (a) hybridizing the target DNA sequence with a control DNA sequence wherein said control DNA sequence is the wild-type DNA sequence corresponding to the target DNA sequence to create a duplex; (b) treating the duplex to remove any spontaneous aldehydes; (c) reacting the duplex with a repair glycosylase to convert any mismatched sites in the duplex to reactive sites containing an aldehyde-containing abasic site; (d) reacting the duplex with a compound of the formula X-Z-Y, wherein X is a detectable moiety, Y is NHNH2, O-NH2 or NH2, and Z is a hydrocarbon, alkylhydroxy, alkylethoxy, alkylester, alkylether, alkylamide or alkylamine, wherein Z may be substituted or unsubstituted; and wherein Z may contain a cleavable group; for a sufficient time and under conditions to covalently bind to the reactive sites; (e) detecting the bound compound to identify sites of mismatches; (f) determining where the mismatch occurs; and (g) determining whether the mismatch is a mutation or polymorphisms.

IPC 1-7 (main, further and additional classification)

C12Q 1/68; C07H 21/04

IPC 8 full level (invention and additional information)

G01N 33/53 (2006.01); C07D 311/16 (2006.01); C07D 311/82 (2006.01); C07D 495/04 (2006.01); C12Q 1/68 (2006.01); G01N 37/00 (2006.01)

CPC (invention and additional information)

C12Q 1/6837 (2013.01); C12Q 1/6827 (2013.01)

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9942622 A1 19990826; AU 2780799 A 19990906; AU 756384 B2 20030109; CA 2321119 A1 19990826; EP 1060266 A1 20001220; EP 1060266 A4 20040922; JP 2002504352 A 20020212

INPADOC legal status


2005-04-06 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20040831

2004-09-22 [A4] SUPPLEMENTARY SEARCH REPORT

- Effective date: 20040809

2000-12-20 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20000918

2000-12-20 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE