EP 1060268 A2 20001220 - METHODS FOR IDENTIFYING POLYCATIONIC, PEPTIDE-LIKE COMPOUNDS WITH ANTIBACTERIAL ACTIVITY
Title (en)
METHODS FOR IDENTIFYING POLYCATIONIC, PEPTIDE-LIKE COMPOUNDS WITH ANTIBACTERIAL ACTIVITY
Title (de)
VERFAHREN ZUR IDENTIFIZIERUNG VON POLYKATIONISCHEN, PEPTIDARTIGEN VERBINDUNGEN MIT ANTIBAKTERIELLEN WIRKUNG
Title (fr)
METHODES D'IDENTIFICATION DE COMPOSES POLYCATIONIQUES, DE TYPE PEPTIDIQUE, A ACTION ANTIBACTERIENNE
Publication
Application
Priority
- US 9904795 W 19990305
- US 7702598 P 19980306
- US 10025798 P 19980914
Abstract (en)
[origin: WO9945152A1] A method is provided for identifying polycationic, peptide-like compounds characterized by antibacterial activity against gram negative bacteria. The method takes advantage of the induction of hyperosomotic stress without leakage of cytoplasmic content. The method involves determining whether the compound has the ability to produce phospholipid exchange betwen the lipid bilayers in conjunction with the induction of the osmotic stress response in the target cell. Determining the induction of the osmotic stress response is effected using a bioluminescent detector organism having a osmY-LUX gene fusion.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12N 15/53 (2006.01); C12Q 1/02 (2006.01); C12Q 1/66 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP)
C12Q 1/6897 (2013.01)
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU NL PT SE
DOCDB simple family (publication)
WO 9945152 A1 19990910; WO 9945152 A8 19991007; AU 2895099 A 19990920; CA 2321931 A1 19990910; EP 1060268 A2 20001220; IL 137934 A0 20011031; JP 2002505120 A 20020219
DOCDB simple family (application)
US 9904795 W 19990305; AU 2895099 A 19990305; CA 2321931 A 19990305; EP 99909832 A 19990305; IL 13793499 A 19990305; JP 2000534683 A 19990305