EP 1064405 A1 20010103 - COMPOSITION CONTAINING DNA CODING FOR A RIBOZYME AND AN OLIGONUCLEOTIDE SUBSTRATE, AND METHOD FOR MEASURING TRANSCRIPTION RATES
Title (en)
COMPOSITION CONTAINING DNA CODING FOR A RIBOZYME AND AN OLIGONUCLEOTIDE SUBSTRATE, AND METHOD FOR MEASURING TRANSCRIPTION RATES
Title (de)
RIBOZYM CODIERENDE DNA UND EIN OLIGONUCLEOTIDSUBSTRAT ENTHALTENDE ZUSAMMENSETZUNG UND VERFAHREN ZUR MESSUNG VON TRANSKRIPTIONSRATEN
Title (fr)
COMPOSITION CONTENANT UN ADN CODANT POUR UN RIBOZYME ET UN SUBSTRAT OLIGONUCLEOTIDIQUE, ET TECHNIQUE PERMETTANT DE MESURER LA VITESSE DE TRANSCRIPTION
Publication
Application
Priority
- DE 19811618 A 19980317
- EP 9901776 W 19990317
Abstract (en)
[origin: WO9947704A1] The invention relates to compositions (reporter systems) containing a DNA sequence coding for a ribozyme as well as an oligonucleotide substrate, which is split by the ribozyme transcribed by the DNA sequence. According to a preferred version of the invention the oligonucleotide substrate is marked with a fluorophoric group (reporter group) and a fluorescence-quenching group (quencher group). After splitting by the ribozyme, quenching of the fluorescence of the fluorophore by the fluorescence-quenching group is suppressed, so that a fluorescence signal is generated. The invention also relates to methods for measuring transcription rates, for example to determine transcription inhibitors or transcription activators.
IPC 1-7
IPC 8 full level
C12N 15/11 (2006.01); C12N 15/113 (2010.01); C12Q 1/68 (2006.01)
CPC (source: EP US)
C12N 15/113 (2013.01 - EP US); C12N 2310/121 (2013.01 - EP US)
Citation (search report)
See references of WO 9947704A1
Designated contracting state (EPC)
CH DE DK ES FR GB IE IT LI NL
DOCDB simple family (publication)
WO 9947704 A1 19990923; DE 19811618 C1 20000831; EP 1064405 A1 20010103; IL 138472 A0 20011031; US 2003008315 A1 20030109; US 6451535 B1 20020917; US 6861223 B2 20050301
DOCDB simple family (application)
EP 9901776 W 19990317; DE 19811618 A 19980317; EP 99915650 A 19990317; IL 13847299 A 19990317; US 21585402 A 20020808; US 64630600 A 20001120