Global Patent Index - EP 1064407 A1

EP 1064407 A1 20010103 - METHOD AND DEVICE FOR DETECTING A NUCLEOTIDE SEQUENCE

Title (en)

METHOD AND DEVICE FOR DETECTING A NUCLEOTIDE SEQUENCE

Title (de)

VERFAHREN UND VORRICHTUNG ZUM NACHWEIS EINER NUKLEOTIDSEQUENZ

Title (fr)

PROCEDE ET DISPOSITIF DE DETECTION D'UNE SEQUENCE NUCLEOTIDIQUE

Publication

EP 1064407 A1 20010103 (DE)

Application

EP 99919084 A 19990316

Priority

  • DE 9900725 W 19990316
  • DE 19811729 A 19980318

Abstract (en)

[origin: DE19811729A1] A method for fluorescent detection of a nucleotide sequence (N) is new in which the presence of N generates or destroys an interaction between two fluorophores (F1, F2), the new feature is that at least one of F1 and F2 is bound to a solid phase (M). The interaction between the fluorophores makes possible a non-radiative (Foerster) or direct energy transfer. Independent claims are also included for the following: (1) a microtiter plate for carrying out the process with F1 bonded to the wells; and (2) kit including the microtiter plate plus an F2-labeled second primer.

IPC 1-7

C12Q 1/68

IPC 8 full level

C12N 15/09 (2006.01); C12M 1/00 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6818 (2018.01)

CPC (source: EP)

C12Q 1/6818 (2013.01)

Citation (search report)

See references of WO 9947700A1

Designated contracting state (EPC)

AT CH DE FR GB LI

DOCDB simple family (publication)

DE 19811729 A1 19990923; DE 19811729 C2 20000518; CA 2323075 A1 19990923; EP 1064407 A1 20010103; JP 2002506654 A 20020305; WO 9947700 A1 19990923

DOCDB simple family (application)

DE 19811729 A 19980318; CA 2323075 A 19990316; DE 9900725 W 19990316; EP 99919084 A 19990316; JP 2000536883 A 19990316