EP 1064407 A1 20010103 - METHOD AND DEVICE FOR DETECTING A NUCLEOTIDE SEQUENCE
Title (en)
METHOD AND DEVICE FOR DETECTING A NUCLEOTIDE SEQUENCE
Title (de)
VERFAHREN UND VORRICHTUNG ZUM NACHWEIS EINER NUKLEOTIDSEQUENZ
Title (fr)
PROCEDE ET DISPOSITIF DE DETECTION D'UNE SEQUENCE NUCLEOTIDIQUE
Publication
Application
Priority
- DE 9900725 W 19990316
- DE 19811729 A 19980318
Abstract (en)
[origin: DE19811729A1] A method for fluorescent detection of a nucleotide sequence (N) is new in which the presence of N generates or destroys an interaction between two fluorophores (F1, F2), the new feature is that at least one of F1 and F2 is bound to a solid phase (M). The interaction between the fluorophores makes possible a non-radiative (Foerster) or direct energy transfer. Independent claims are also included for the following: (1) a microtiter plate for carrying out the process with F1 bonded to the wells; and (2) kit including the microtiter plate plus an F2-labeled second primer.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12M 1/00 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6818 (2018.01)
CPC (source: EP)
C12Q 1/6818 (2013.01)
Citation (search report)
See references of WO 9947700A1
Designated contracting state (EPC)
AT CH DE FR GB LI
DOCDB simple family (publication)
DE 19811729 A1 19990923; DE 19811729 C2 20000518; CA 2323075 A1 19990923; EP 1064407 A1 20010103; JP 2002506654 A 20020305; WO 9947700 A1 19990923
DOCDB simple family (application)
DE 19811729 A 19980318; CA 2323075 A 19990316; DE 9900725 W 19990316; EP 99919084 A 19990316; JP 2000536883 A 19990316