Global Patent Index - EP 1066368 A1

EP 1066368 A1 2001-01-10 - SELECTIVE CELL ANALYSIS

Title (en)

SELECTIVE CELL ANALYSIS

Title (de)

SELEKTIVE ANALYSE VON ZELLEN

Title (fr)

ANALYSE SELECTIVE DE CELLULES

Publication

EP 1066368 A1 (EN)

Application

EP 99909695 A

Priority

  • US 9904415 W
  • US 3296498 A
  • US 9425698 A

Abstract (en)

[origin: WO9945094A1] A method and device for the laser scanning analysis of a selected small portion of specimen cells in a stationary and very localized area of a specimen slide, which cells are primarily specifically selected to be of scanning interest. The specimen may be pretreated with an adhering medium specifically tailored to characteristics of the selected cells whereby specific cells are made adherent thereby. The specimen slide is then locally provided with a co-acting adhering material or adhering device, or filtering device in a non-impeding gate position, whereby a statistically significant number of selected cells do not reach or are adhered to the gate position and remaining non-selected cells are removed from the gate position. It is the gate position which is then scanned by the laser scanning cytometer with increased efficiency. The adhering materials are either coated onto the surface of the gating area or are provided with magnetically susceptible particles. In the latter embodiment, a localized magnet is placed adjacent the gating area to adhere magnetized selected cells to the gating area. The cells of interest may be a cell class or set having a subset (e.g., white blood cells and monocytes), with characteristics of the subset being of analytic interest. Accordingly, initial reagents are used for the adhering and additional reagents are used for marking the subset and analyzing the desired characteristics.

IPC 1-7 (main, further and additional classification)

C12M 1/00; C12M 1/12; C12Q 1/00; C12Q 1/06; C12Q 1/24; C12Q 1/68; G01N 33/543; G01N 33/569

IPC 8 full level (invention and additional information)

G01N 33/531 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12Q 1/02 (2006.01); C12Q 1/06 (2006.01); C12Q 1/24 (2006.01); G01N 1/28 (2006.01); G01N 15/06 (2006.01); G01N 15/14 (2006.01); G01N 33/48 (2006.01); G01N 33/50 (2006.01); G01N 33/543 (2006.01); G01N 33/569 (2006.01); G01N 15/00 (2006.01); G01N 15/04 (2006.01)

CPC (invention and additional information)

G01N 15/1456 (2013.01); G01N 15/0625 (2013.01); G01N 33/5005 (2013.01); G01N 15/042 (2013.01); G01N 2015/0084 (2013.01); G01N 2015/1465 (2013.01); G01N 2015/1486 (2013.01)

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9945094 A1 19990910; AU 2884499 A 19990920; CA 2321203 A1 19990910; EP 1066368 A1 20010110; EP 1066368 A4 20020821; JP 2002505441 A 20020219

INPADOC legal status


2002-11-06 [18W] WITHDRAWN

- Date of withdrawal: 20020910

2002-08-21 [A4] SUPPLEMENTARY SEARCH REPORT

- Effective date: 20020709

2002-08-21 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A4

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2002-08-14 [RIC1] CLASSIFICATION (CORRECTION)

- Free text: 7C 12M 1/00 A, 7C 12M 1/12 B, 7C 12Q 1/00 B, 7C 12Q 1/06 B, 7C 12Q 1/24 B, 7C 12Q 1/68 B, 7G 01N 33/543 B, 7G 01N 33/569 B, 7G 01N 15/14 B

2001-01-10 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20001002

2001-01-10 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2001-01-10 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO

- Free text: AL PAYMENT 20001002;LT PAYMENT 20001002;LV PAYMENT 20001002;MK PAYMENT 20001002;RO PAYMENT 20001002;SI PAYMENT 20001002