Global Patent Index - EP 1066391 A2

EP 1066391 A2 2001-01-10 - ANTIGEN PREPARATION AND USE

Title (en)

ANTIGEN PREPARATION AND USE

Title (de)

FUSIONSPROTEIN ENTHALTEND EINE SEQUENZ DES HAUPT-HÜLLPROTEINS EINES PAPOVAVIRUS

Title (fr)

PREPARATION ET UTILISATION D'ANTIGENES

Publication

EP 1066391 A2 (EN)

Application

EP 99913478 A

Priority

  • GB 9900978 W
  • GB 9806666 A

Abstract (en)

[origin: WO9950424A2] Modified virus-like particles (VLPs) can comprise fusion proteins having sequence from a major coat protein of a papovavirus, e.g. L1 protein of HPV 16 or 18, in which the N-terminal of the sequence derived from the major coat protein is fused to a further peptide sequence. The VLPs can contain a full sequence of an L1 protein, or an L1 sequence with an N-terminal deletion, or an L1 sequence with an aminoacid substitution mutation, and optionally a C-terminal L1 sequence deletion. The peptide sequence fused to the N-terminal can be immunogenic e.g. from a protein of a pathogen such as a virus. The further peptide sequence can provide a binding domain for affinity purification of the VLP. Modified VLPs can retain the native conformation of the VLP structure while also presenting to the immune system of a subject immunised with the modified VLPs an epitope present on an N-terminal extension of the major coat protein sequence. Corresponding polynucleotides, expression vectors, plasmids, vectors and cells containing such polynucleotides are disclosed.

IPC 1-7 (main, further and additional classification)

C12N 15/62; A61K 39/12; C07K 14/025; C07K 19/00; C12N 5/10; C12N 7/01; C12N 7/02

IPC 8 full level (invention and additional information)

C07K 14/025 (2006.01); C12N 7/04 (2006.01); A61K 39/00 (2006.01)

CPC (invention and additional information)

C07K 14/005 (2013.01); C12N 7/00 (2013.01); A61K 39/00 (2013.01); C07K 2319/00 (2013.01); C12N 2710/14143 (2013.01); C12N 2710/20022 (2013.01); C12N 2710/20023 (2013.01)

Citation (search report)

See references of WO 9950424A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9950424 A2 19991007; WO 9950424 A3 19991202; AU 3159499 A 19991018; EP 1066391 A2 20010110; GB 9806666 D0 19980527

INPADOC legal status


2004-12-15 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20040608

2004-01-07 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20031125

2001-01-10 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20001013

2001-01-10 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE