EP 1066391 A2 20010110 - ANTIGEN PREPARATION AND USE
Title (en)
ANTIGEN PREPARATION AND USE
Title (de)
FUSIONSPROTEIN ENTHALTEND EINE SEQUENZ DES HAUPT-HÜLLPROTEINS EINES PAPOVAVIRUS
Title (fr)
PREPARATION ET UTILISATION D'ANTIGENES
Publication
Application
Priority
- GB 9900978 W 19990329
- GB 9806666 A 19980327
Abstract (en)
[origin: WO9950424A2] Modified virus-like particles (VLPs) can comprise fusion proteins having sequence from a major coat protein of a papovavirus, e.g. L1 protein of HPV 16 or 18, in which the N-terminal of the sequence derived from the major coat protein is fused to a further peptide sequence. The VLPs can contain a full sequence of an L1 protein, or an L1 sequence with an N-terminal deletion, or an L1 sequence with an aminoacid substitution mutation, and optionally a C-terminal L1 sequence deletion. The peptide sequence fused to the N-terminal can be immunogenic e.g. from a protein of a pathogen such as a virus. The further peptide sequence can provide a binding domain for affinity purification of the VLP. Modified VLPs can retain the native conformation of the VLP structure while also presenting to the immune system of a subject immunised with the modified VLPs an epitope present on an N-terminal extension of the major coat protein sequence. Corresponding polynucleotides, expression vectors, plasmids, vectors and cells containing such polynucleotides are disclosed.
IPC 1-7
C12N 15/62; C12N 5/10; C12N 7/01; C12N 7/02; C07K 14/025; C07K 19/00; A61K 39/12
IPC 8 full level
C07K 14/025 (2006.01); C12N 7/04 (2006.01); A61K 39/00 (2006.01)
CPC (source: EP)
C07K 14/005 (2013.01); C12N 7/00 (2013.01); A61K 39/00 (2013.01); C07K 2319/00 (2013.01); C12N 2710/14143 (2013.01); C12N 2710/20022 (2013.01); C12N 2710/20023 (2013.01)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9950424 A2 19991007; WO 9950424 A3 19991202; AU 3159499 A 19991018; EP 1066391 A2 20010110; GB 9806666 D0 19980527
DOCDB simple family (application)
GB 9900978 W 19990329; AU 3159499 A 19990329; EP 99913478 A 19990329; GB 9806666 A 19980327