Global Patent Index - EP 1066409 A1

EP 1066409 A1 2001-01-10 - METHOD FOR DETECTING DAMAGED DNA USING PROTEIN COMPLEXES AND ELEMENTS FOR IMPLEMENTING SAID METHOD

Title (en)

METHOD FOR DETECTING DAMAGED DNA USING PROTEIN COMPLEXES AND ELEMENTS FOR IMPLEMENTING SAID METHOD

Title (de)

VERFAHREN ZUR ERKENNUNG VON DNA-SCHÄDEN MITTELS PROTEINKOMPLEXEN SOWIE HILFSMITTEL ZUR DURCHFÜHRUNG DIESES VERFAHRENS

Title (fr)

PROCEDE DE DETECTION DE LESIONS DE L'ADN AU MOYEN DE COMPLEXES DE PROTEINES ET ELEMENTS PERMETTANT LA MISE EN OEUVRE DU PROCEDE

Publication

EP 1066409 A1 (FR)

Application

EP 99910424 A

Priority

  • FR 9900691 W
  • FR 9804064 A

Abstract (en)

[origin: FR2776670A1] The invention concerns a method for qualitatively and quantitatively detecting DNA lesions comprising the following different steps: preparing DNA; subjecting said DNA to damaging treatment; and fixing said damaged DNA on a sensitised solid support; or preparing DNA; fixing said non-damaged DNA on a sensitised solid support; and subjecting said DNA to damaging treatment; or treating cells; lysis and capture of cell DNA. The invention is characterised in that it consists in: treating said damaged DNA with a composition comprising a cell extract or a purified protein having at least an activity for recognising and/or repairing lesions; and detecting on the damaged DNA, directly or indirectly, the presence of the proteins recognising and/or repairing the resulting lesions; a washing step intervening between all the different steps. The invention also concerns the elements for implementing said method.

IPC 1-7 (main, further and additional classification)

C12Q 1/68

IPC 8 full level (invention and additional information)

C12Q 1/68 (2006.01)

CPC (invention and additional information)

C12Q 1/6827 (2013.01)

Combination set (CPC)

C12Q 1/6827 + C12Q 2563/131 + C12Q 2545/114 + C12Q 2521/514

Citation (search report)

See references of WO 9949080A1

Designated contracting state (EPC)

BE CH DE FR GB IE IT LI NL PT SE

EPO simple patent family

FR 2776670 A1 19991001; FR 2776670 B1 20001208; EP 1066409 A1 20010110; WO 9949080 A1 19990930

INPADOC legal status


2004-04-14 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20030930

2002-05-22 [RAP1] TRANSFER OF RIGHTS OF AN EP PUBLISHED APPLICATION

- Owner name: SFRI DIAGNOSTICS

2001-01-10 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20001018

2001-01-10 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): BE CH DE FR GB IE IT LI NL PT SE