EP 1066409 A1 20010110 - METHOD FOR DETECTING DAMAGED DNA USING PROTEIN COMPLEXES AND ELEMENTS FOR IMPLEMENTING SAID METHOD
Title (en)
METHOD FOR DETECTING DAMAGED DNA USING PROTEIN COMPLEXES AND ELEMENTS FOR IMPLEMENTING SAID METHOD
Title (de)
VERFAHREN ZUR ERKENNUNG VON DNA-SCHÄDEN MITTELS PROTEINKOMPLEXEN SOWIE HILFSMITTEL ZUR DURCHFÜHRUNG DIESES VERFAHRENS
Title (fr)
PROCEDE DE DETECTION DE LESIONS DE L'ADN AU MOYEN DE COMPLEXES DE PROTEINES ET ELEMENTS PERMETTANT LA MISE EN OEUVRE DU PROCEDE
Publication
Application
Priority
- FR 9900691 W 19990325
- FR 9804064 A 19980326
Abstract (en)
[origin: FR2776670A1] The invention concerns a method for qualitatively and quantitatively detecting DNA lesions comprising the following different steps: preparing DNA; subjecting said DNA to damaging treatment; and fixing said damaged DNA on a sensitised solid support; or preparing DNA; fixing said non-damaged DNA on a sensitised solid support; and subjecting said DNA to damaging treatment; or treating cells; lysis and capture of cell DNA. The invention is characterised in that it consists in: treating said damaged DNA with a composition comprising a cell extract or a purified protein having at least an activity for recognising and/or repairing lesions; and detecting on the damaged DNA, directly or indirectly, the presence of the proteins recognising and/or repairing the resulting lesions; a washing step intervening between all the different steps. The invention also concerns the elements for implementing said method.
IPC 1-7
IPC 8 full level
C12Q 1/68 (2006.01); C12Q 1/6827 (2018.01)
CPC (source: EP)
C12Q 1/6827 (2013.01)
Citation (search report)
See references of WO 9949080A1
Designated contracting state (EPC)
BE CH DE FR GB IE IT LI NL PT SE
DOCDB simple family (publication)
FR 2776670 A1 19991001; FR 2776670 B1 20001208; EP 1066409 A1 20010110; WO 9949080 A1 19990930
DOCDB simple family (application)
FR 9804064 A 19980326; EP 99910424 A 19990325; FR 9900691 W 19990325