EP 1066523 A2 20010110 - CONFIRMATORY ASSAYS FOR SMALL MOLECULE DRUGS
Title (en)
CONFIRMATORY ASSAYS FOR SMALL MOLECULE DRUGS
Title (de)
KONTROLLBESTIMMUNGSMETHODEN FÜR DROGEN BESTEHEND AUS KLEINEN MOLEKÜLEN
Title (fr)
DOSAGES CONFIRMATIFS DE DROGUES CONSTITUEES DE MOLECULES DE TAILLE REDUITE
Publication
Application
Priority
US 9806098 W 19980327
Abstract (en)
[origin: WO9826644A2] The invention relates to an assay system for the improved detection of analytes, and the ability to distinguish them from cross-reacting substances. Samples giving a positive reaction in a direct assay test are treated with a neutralizing antibody that inhibits reactivity of the true analyte, but not the interfering substance. In bidirectional antibody type confirmatory assays, the neutralizing antibody binds the analyte from a different orientation, and does not impede other reagents in the assay mixture. In adsorption type confirmatory assays, the neutralizing antibody is provided in an amount sufficient to adsorb the analyte but not all of the interfering substance. When retested in an immunoassay, the neutralized sample gives a negative result if it originally contained the true analyte. Samples giving a positive reaction in both the direct and confirmatory tests are marked as containing an interfering substance. The confirmatory assay easily distinguishes the true analyte and reduces the rate of false positives, even when the interfering substance is unknown and present at high concentration.
IPC 1-7
IPC 8 full level
G01N 33/53 (2006.01); G01N 33/543 (2006.01)
CPC (source: EP)
G01N 33/53 (2013.01); G01N 33/54306 (2013.01)
Citation (search report)
See references of WO 9826644A2
Designated contracting state (EPC)
DE GB
DOCDB simple family (publication)
WO 9826644 A2 19980625; WO 9826644 A3 19990218; WO 9826644 A9 19990408; DE 1066523 T1 20010920; EP 1066523 A2 20010110
DOCDB simple family (application)
US 9806098 W 19980327; DE 98915179 T 19980327; EP 98915179 A 19980327