Global patent index - EP 1066523 A2

EP 1066523 A2 2001-01-10 - CONFIRMATORY ASSAYS FOR SMALL MOLECULE DRUGS

Title (en)

CONFIRMATORY ASSAYS FOR SMALL MOLECULE DRUGS

Title (de)

KONTROLLBESTIMMUNGSMETHODEN FÜR DROGEN BESTEHEND AUS KLEINEN MOLEKÜLEN

Title (fr)

DOSAGES CONFIRMATIFS DE DROGUES CONSTITUEES DE MOLECULES DE TAILLE REDUITE

Publication

EP 1066523 A2 (EN)

Application

EP 98915179 A

Priority

US 9806098 W

Abstract (en)

[origin: WO9826644A2] The invention relates to an assay system for the improved detection of analytes, and the ability to distinguish them from cross-reacting substances. Samples giving a positive reaction in a direct assay test are treated with a neutralizing antibody that inhibits reactivity of the true analyte, but not the interfering substance. In bidirectional antibody type confirmatory assays, the neutralizing antibody binds the analyte from a different orientation, and does not impede other reagents in the assay mixture. In adsorption type confirmatory assays, the neutralizing antibody is provided in an amount sufficient to adsorb the analyte but not all of the interfering substance. When retested in an immunoassay, the neutralized sample gives a negative result if it originally contained the true analyte. Samples giving a positive reaction in both the direct and confirmatory tests are marked as containing an interfering substance. The confirmatory assay easily distinguishes the true analyte and reduces the rate of false positives, even when the interfering substance is unknown and present at high concentration.

IPC 1-7 (main, further and additional classification)

G01N 33/542; G01N 33/94

IPC 8 full level (invention and additional information)

G01N 33/53 (2006.01); G01N 33/543 (2006.01)

CPC (invention and additional information)

G01N 33/54306 (2013.01); G01N 33/53 (2013.01)

Citation (search report)

See references of WO 9826644A3

Designated contracting state (EPC)

DE GB

EPO simple patent family

WO 9826644 A2 19980625; WO 9826644 A3 19990218; WO 9826644 A9 19990408; DE 1066523 T1 20010920; EP 1066523 A2 20010110

INPADOC legal status

2001-09-20 [DET] DE: TRANSLATION OF PATENT CLAIMS

- Ref Legal Event Code: DET

2001-08-22 [18W] WITHDRAWN

- Ref Legal Event Code: 18W

- Date of withdrawal: 20010628

2001-01-10 [17P] REQUEST FOR EXAMINATION FILED

- Ref Legal Event Code: 17P

- Effective date: 20001025

2001-01-10 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): DE GB