EP 1068330 A2 20010117 - RECOMBINANT POTYVIRUS CONSTRUCT AND USE THEREOF
Title (en)
RECOMBINANT POTYVIRUS CONSTRUCT AND USE THEREOF
Title (de)
REKOMBINANTES POTYVIRUS-KONSTRUKT UND DESSEN VERWENDUNG
Title (fr)
POTYVIRUS RECOMBINANT ET UTILISATION DE CE DERNIER
Publication
Application
Priority
- IL 9900184 W 19990330
- IL 12399498 A 19980407
Abstract (en)
[origin: WO9951749A2] The present invention relates to a recombinant potyvirus infectious nucleic acid, preferably cDNA or RNA, construct useful for plant cross protection, comprising a full length clone characterized in that its HC-Pro gene conserved FRNK box sequence contains a substitution, preferably a substitution of Arg. Preferably, the construct further contains a substitution which effectively abolishes aphid transmissibility. The present invention further relates to a method for the production of a mild strain of potyvirus utilizing the above mentioned construct and to a method for protecting plants from viral infection and for transient expression of foreign nucleic acid (genes) in plants, using said construct. Preferably, the present invention relates to a method for cross protection of curcurbits against ZYMV infection. The present invention also relates to a method for providing protection against viral infection in plants comprising inoculating plants, by mechanical inoculation or by bombardment, with the recombinant potyvirus infectious construct.
IPC 1-7
C12N 15/40; C12N 15/57; C12N 15/82; C12N 15/83; C12N 7/04; C12N 7/00; A01N 63/02
IPC 8 full level
A01H 1/00 (2006.01); A01N 63/00 (2006.01); A01N 63/02 (2006.01); C07K 14/08 (2006.01); C12N 7/00 (2006.01); C12N 7/04 (2006.01); C12N 9/50 (2006.01); C12N 15/09 (2006.01); C12N 15/40 (2006.01); C12N 15/82 (2006.01); C12N 15/83 (2006.01)
CPC (source: EP US)
A01N 63/60 (2020.01 - EP US); C07K 14/005 (2013.01 - EP US); C12N 9/506 (2013.01 - EP); C12N 15/8203 (2013.01 - EP); C12N 15/8283 (2013.01 - EP); C12N 2770/34022 (2013.01 - EP)
Citation (search report)
See references of WO 9951749A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9951749 A2 19991014; WO 9951749 A3 19991209; AU 3052599 A 19991025; CA 2327445 A1 19991014; CN 1303433 A 20010711; EP 1068330 A2 20010117; JP 2002536956 A 20021105; TR 200002909 T2 20010221
DOCDB simple family (application)
IL 9900184 W 19990330; AU 3052599 A 19990330; CA 2327445 A 19990330; CN 99806606 A 19990330; EP 99912037 A 19990330; JP 2000542461 A 19990330; TR 200002909 T 19990330