Global Patent Index - EP 1068330 A2

EP 1068330 A2 2001-01-17 - RECOMBINANT POTYVIRUS CONSTRUCT AND USE THEREOF

Title (en)

RECOMBINANT POTYVIRUS CONSTRUCT AND USE THEREOF

Title (de)

REKOMBINANTES POTYVIRUS-KONSTRUKT UND DESSEN VERWENDUNG

Title (fr)

POTYVIRUS RECOMBINANT ET UTILISATION DE CE DERNIER

Publication

EP 1068330 A2 (EN)

Application

EP 99912037 A

Priority

  • IL 9900184 W
  • IL 12399498 A

Abstract (en)

[origin: WO9951749A2] The present invention relates to a recombinant potyvirus infectious nucleic acid, preferably cDNA or RNA, construct useful for plant cross protection, comprising a full length clone characterized in that its HC-Pro gene conserved FRNK box sequence contains a substitution, preferably a substitution of Arg. Preferably, the construct further contains a substitution which effectively abolishes aphid transmissibility. The present invention further relates to a method for the production of a mild strain of potyvirus utilizing the above mentioned construct and to a method for protecting plants from viral infection and for transient expression of foreign nucleic acid (genes) in plants, using said construct. Preferably, the present invention relates to a method for cross protection of curcurbits against ZYMV infection. The present invention also relates to a method for providing protection against viral infection in plants comprising inoculating plants, by mechanical inoculation or by bombardment, with the recombinant potyvirus infectious construct.

IPC 1-7 (main, further and additional classification)

C12N 15/40; A01N 63/02; C12N 7/00; C12N 7/04; C12N 15/57; C12N 15/82; C12N 15/83

IPC 8 full level (invention and additional information)

A01H 1/00 (2006.01); A01N 63/00 (2006.01); A01N 63/02 (2006.01); C07K 14/08 (2006.01); C12N 7/00 (2006.01); C12N 7/04 (2006.01); C12N 9/50 (2006.01); C12N 15/09 (2006.01); C12N 15/40 (2006.01); C12N 15/82 (2006.01); C12N 15/83 (2006.01)

CPC (invention and additional information)

C07K 14/005 (2013.01); A01N 63/00 (2013.01); A01N 63/02 (2013.01); C12N 9/506 (2013.01); C12N 15/8203 (2013.01); C12N 15/8283 (2013.01); C12N 2770/34022 (2013.01)

Citation (search report)

See references of WO 9951749A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9951749 A2 19991014; WO 9951749 A3 19991209; AU 3052599 A 19991025; CA 2327445 A1 19991014; CN 1303433 A 20010711; EP 1068330 A2 20010117; JP 2002536956 A 20021105; TR 200002909 T2 20010221

INPADOC legal status


2005-04-20 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20041001

2004-02-11 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20031230

2001-01-17 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20001005

2001-01-17 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE