EP 1070127 A1 20010124 - DNA SEQUENCE IN THE Sp5 MUTANT AND SITE SPECIFIC GENETIC RECOMBINATION
Title (en)
DNA SEQUENCE IN THE Sp5 MUTANT AND SITE SPECIFIC GENETIC RECOMBINATION
Title (de)
DNA SEQUENZ IN DER SP5 MUTANTE UND ORTSGERICHTETE GENETISCHE REKOMBINATION
Title (fr)
SEQUENCE D'ADN CHEZ LE MUTANT Sp5 ET RECOMBINAISON GENETIQUE SPECIFIQUE AU SITE
Publication
Application
Priority
- SE 9900573 W 19990408
- SE 9801245 A 19980408
Abstract (en)
[origin: WO9953048A1] This invention relates to a DNA sequence of 956 base pairs as given in Figure 2, sequence list 1 and to a DNA sequence including two repetitive sequences consisting of ATTGATTAT and ATTATTAT as repeat units separated by a spacer of one base pair at 5' end and of two TGTATAATA as repeat units separated by a spacer of one base pair at 3' end. It also relates to methods for a site specific recombinogenic process, to a method for introducing a gene of interest into mammalian cells and a method for removal of sequences previously introduced by any gene targeting method. The use of 5'-TCTTT T TCTTT-3' for recombination in mammalian cells and the use of a direct repeat sequence of 10 bases and one base spacer, 5'-TCTTT T TCTTT-3' for transgenic work and as for a tool of site specific recombination are also claimed.
IPC 1-7
IPC 8 full level
C12N 9/10 (2006.01); C12N 15/10 (2006.01)
CPC (source: EP)
C12N 9/1048 (2013.01); C12N 15/10 (2013.01)
Citation (search report)
See references of WO 9953048A1
Designated contracting state (EPC)
CH DE FR GB IT LI NL SE
DOCDB simple family (publication)
WO 9953048 A1 19991021; AU 4066199 A 19991101; EP 1070127 A1 20010124; SE 9801245 D0 19980408
DOCDB simple family (application)
SE 9900573 W 19990408; AU 4066199 A 19990408; EP 99924074 A 19990408; SE 9801245 A 19980408