Global Patent Index - EP 1070127 A1

EP 1070127 A1 2001-01-24 - DNA SEQUENCE IN THE Sp5 MUTANT AND SITE SPECIFIC GENETIC RECOMBINATION

Title (en)

DNA SEQUENCE IN THE Sp5 MUTANT AND SITE SPECIFIC GENETIC RECOMBINATION

Title (de)

DNA SEQUENZ IN DER SP5 MUTANTE UND ORTSGERICHTETE GENETISCHE REKOMBINATION

Title (fr)

SEQUENCE D'ADN CHEZ LE MUTANT Sp5 ET RECOMBINAISON GENETIQUE SPECIFIQUE AU SITE

Publication

EP 1070127 A1 (EN)

Application

EP 99924074 A

Priority

  • SE 9900573 W
  • SE 9801245 A

Abstract (en)

[origin: WO9953048A1] This invention relates to a DNA sequence of 956 base pairs as given in Figure 2, sequence list 1 and to a DNA sequence including two repetitive sequences consisting of ATTGATTAT and ATTATTAT as repeat units separated by a spacer of one base pair at 5' end and of two TGTATAATA as repeat units separated by a spacer of one base pair at 3' end. It also relates to methods for a site specific recombinogenic process, to a method for introducing a gene of interest into mammalian cells and a method for removal of sequences previously introduced by any gene targeting method. The use of 5'-TCTTT T TCTTT-3' for recombination in mammalian cells and the use of a direct repeat sequence of 10 bases and one base spacer, 5'-TCTTT T TCTTT-3' for transgenic work and as for a tool of site specific recombination are also claimed.

IPC 1-7 (main, further and additional classification)

C12N 15/10; C12N 15/11

IPC 8 full level (invention and additional information)

C12N 9/10 (2006.01); C12N 15/10 (2006.01)

CPC (invention and additional information)

C12N 15/10 (2013.01); C12N 9/1048 (2013.01)

Citation (search report)

See references of WO 9953048A1

Designated contracting state (EPC)

CH DE FR GB IT LI NL SE

EPO simple patent family

WO 9953048 A1 19991021; AU 4066199 A 19991101; EP 1070127 A1 20010124; SE 9801245 D0 19980408

INPADOC legal status


2003-05-28 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20021101

2001-01-24 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20001108

2001-01-24 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): CH DE FR GB IT LI NL SE