Global Patent Index - EP 1070145 A1

EP 1070145 A1 2001-01-24 - METHODS FOR IDENTIFYING GENETIC DETERMINANTS ASSOCIATED WITH MODULATION OF TEST COMPOUND ACTIVITY

Title (en)

METHODS FOR IDENTIFYING GENETIC DETERMINANTS ASSOCIATED WITH MODULATION OF TEST COMPOUND ACTIVITY

Title (de)

VERFAHREN ZUR IDENTIFIZIERUNG VON GENETISCHEN DETERMINANTEN ASSOZIERT MIT MODULATION DER ACTIVITÄT VON PRÜF ZUSAMMENSETZUNGEN

Title (fr)

PROCEDES D'IDENTIFICATION DE DETERMINANTS GENETIQUES ASSOCIES A LA MODULATION DE L'ACTIVITE DE COMPOSES DE TEST

Publication

EP 1070145 A1 (EN)

Application

EP 99918480 A

Priority

  • US 9907802 W
  • US 8122498 P

Abstract (en)

[origin: WO9953098A1] Disclosed are methods for identifying genetic determinants that modulate the activity of test compounds which affect detectable processes in eukaryotic cells. The process comprises exposing a first cell, whether prokaryotic or eukaryotic, to a test compound to determine if the test compound modulates the detectable process in the cell. If so, the compound is then exposed to a second pool of cells (preferably of the same type as the first pool of cells) which overexpress one or more heterologous polypeptides under conditions which, in the absence of the compound, allow the detectable process to occur. If overexpression of the heterologous polypeptide alters modulation of the detectable process, such modulation allows the detectable process to be correlated with the test compound or with a particular compound and the overexpressed heterologous polypeptide, and the gene encoding the same. Also described are methods for correlating a gene with a detectable cellular process, wherein a first pool of cells, whether prokaryotic or eukaryotic, is exposed to a test compound, determining if the test compound modulates a detectable process in the cells, and if so, exposing the compound to a second pool of cells (preferably of the same type as the first pool of cells) which overexpress a heterologous polypeptide. If overexpression of the heterologous polypeptide alters modulation of the detectable process in the cells by the compound, the heterologous polypeptide responsible for such modulation, and preferably the nucleic acid molecule encoding the same, are characterized and correlated with the detectable process.

IPC 1-7 (main, further and additional classification)

C12Q 1/68; C12N 15/10

IPC 8 full level (invention and additional information)

C12N 15/10 (2006.01); C12Q 1/68 (2006.01)

CPC (invention and additional information)

C12N 15/1072 (2013.01); C12N 15/1048 (2013.01); C12Q 1/6897 (2013.01)

Citation (search report)

See references of WO 9953098A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9953098 A1 19991021; CA 2327830 A1 19991021; EP 1070145 A1 20010124

INPADOC legal status


2004-05-12 [18D] DEEMED TO BE WITHDRAWN

- Ref Legal Event Code: 18D

- Effective date: 20031104

2001-01-24 [17P] REQUEST FOR EXAMINATION FILED

- Ref Legal Event Code: 17P

- Effective date: 20001107

2001-01-24 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE