Global Patent Index - EP 1071472 A4

EP 1071472 A4 20020417 - PEPTIDES FOR EFFICIENT GENE TRANSFER

Title (en)

PEPTIDES FOR EFFICIENT GENE TRANSFER

Title (de)

PEPTIDE FÜR EINEN EFFIZIENTEN GENTRANSFER

Title (fr)

PEPTIDES PERMETTANT UN TRANSFERT GENIQUE EFFICACE

Publication

EP 1071472 A4 20020417 (EN)

Application

EP 99919987 A 19990423

Priority

  • US 9908884 W 19990423
  • US 6589198 A 19980423

Abstract (en)

[origin: WO9953961A1] The present invention relates to nucleic acid condensates comprising a nucleic acid bound to a polycationic peptide, and in particular a CWK (cysteine, tryptophan, lysine) polycationic peptide, and to methods of making and using such condensates. The invention further relates to a novel pharmaceutical compositions comprising condensed DNA incorporated into matrices (gene-activated matrices) that may be utilized for delivery of nucleic acids into targeted cells. The invention further relates to methods for producing gene-activated matrices involving the addition of polycationic peptides, and in particular a CWK polycationic peptide, to negatively charged DNA prior to incorporation into a matrix. The invention further relates to the linkage of the polycationic peptides to ligand molecules, thus permitting targeting of the DNA to specific targeted cell types. The present invention provides pharmaceutical formulations and methods that are applicable to wound healing and a wide variety of genetic or acquired diseases.

IPC 1-7

A61K 48/00; C12N 15/00; C12N 15/11; C12N 15/63; C12N 15/85; C12N 15/86

IPC 8 full level

A61K 48/00 (2006.01); A61P 17/02 (2006.01); C07K 14/00 (2006.01); C12N 15/87 (2006.01); A61K 38/00 (2006.01)

CPC (source: EP)

A61K 48/00 (2013.01); A61P 17/02 (2017.12); C07K 14/001 (2013.01); C12N 15/87 (2013.01); A61K 38/00 (2013.01); C07K 2319/00 (2013.01)

Citation (search report)

  • [PX] WO 9819711 A1 19980514 - UNIV MICHIGAN [US], et al
  • [Y] WO 9746100 A1 19971211 - UNIV CASE WESTERN RESERVE [US]
  • [XY] SMITH L C ET AL: "SYNTHETIC PEPTIDE-BASED DNA COMPLEXES FOR NONVIRAL GENE DELIVERY", ADVANCED DRUG DELIVERY REVIEWS, AMSTERDAM, NL, vol. 30, no. 1-3, 2 March 1998 (1998-03-02), pages 115 - 131, XP000990852, ISSN: 0169-409X
  • [PX] ADAMI ROGER C ET AL: "Stability of peptide-condensed plasmid DNA formulations", JOURNAL OF PHARMACEUTICAL SCIENCES, AMERICAN PHARMACEUTICAL ASSOCIATION. WASHINGTON, US, vol. 87, no. 6, June 1998 (1998-06-01), pages 678 - 683, XP002165043, ISSN: 0022-3549
  • [Y] WOLFERT M A ET AL: "CHARACTERIZATION OF VECTORS FOR GENE THERAPY FORMED BY SELF- ASSEMBLY OF DNA WITH SYNTHETIC BLOCK CO-POLYMERS", HUMAN GENE THERAPY, XX, XX, vol. 7, 10 November 1996 (1996-11-10), pages 2123 - 2133, XP002915739, ISSN: 1043-0342
  • [A] KWOK KY ET AL: "Formulation of highly soluble poly(ethylene glycol)-peptide DNA condensates", JOURNAL OF PHARMACEUTICAL SCIENCES, AMERICAN PHARMACEUTICAL ASSOCIATION. WASHINGTON, US, vol. 88, no. 10, October 1999 (1999-10-01), pages 996 - 1003, XP002165042, ISSN: 0022-3549
  • See references of WO 9953961A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 9953961 A1 19991028; WO 9953961 A9 20000908; AU 3758199 A 19991108; EP 1071472 A1 20010131; EP 1071472 A4 20020417

DOCDB simple family (application)

US 9908884 W 19990423; AU 3758199 A 19990423; EP 99919987 A 19990423