Global Patent Index - EP 1071785 A2

EP 1071785 A2 2001-01-31 - INTERCONVERSION OF PLANT FATTY ACID DESATURASES AND HYDROXYLASES

Title (en)

INTERCONVERSION OF PLANT FATTY ACID DESATURASES AND HYDROXYLASES

Title (de)

UMWANDLUNG VON PFLANLICHEN FETTSÄURE-DESATURASEN UND HYDROXYLASEN

Title (fr)

INTERCONVERSION DE DESATURASES ET D'HYDROXYLASES D'ACIDES GRAS VEGETAUX

Publication

EP 1071785 A2 (EN)

Application

EP 99921394 A

Priority

  • US 9908400 W
  • US 8193698 P
  • US 12316899 P

Abstract (en)

[origin: WO9953073A2] A method is provided for modifying a fatty acyl desaturase to a fatty acyl hydroxylase consisting of identifying and changing as few as four amino acid residues that are conserved in functionally equivalent desaturase enzymes from various plant species but that are not identical in fatty acyl hydroxylases that exhibit significant overall sequence similarity to the fatty acyl desaturases, and which catalyze hydroxylation at one of the carbon residues on the fatty acyl substrate that is desaturated by the corresponding desaturase; the modifications being made by changing the amino acid residue so that it is identical or functionally equivalent to the amino acid residue found in the naturally occurring hydroxylase. Also provided is a similar method of modifying a fatty acyl hydroxylase to a fatty acyl desaturase by changing seven or fewer amino acid residues. Transgenic plants and products of such transgenic plants wherein the plants have been modified to produce a modified hydroxylase or desaturase are also provided.

IPC 1-7 (main, further and additional classification)

C12N 15/53; A01H 5/00; C12N 9/02; C12N 15/82

IPC 8 full level (invention and additional information)

C12N 9/02 (2006.01); C12N 15/53 (2006.01); C12N 15/82 (2006.01)

CPC (invention and additional information)

C12N 9/0071 (2013.01); C12N 9/0083 (2013.01); C12N 15/8247 (2013.01)

Citation (search report)

See references of WO 9953073A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9953073 A2 19991021; WO 9953073 A3 20000210; WO 9953073 A9 20000720; AU 3862099 A 19991101; BR 9910125 A 20030225; CA 2326687 A1 19991021; EP 1071785 A2 20010131; IL 139062 D0 20011125

INPADOC legal status


2008-04-30 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20071101

2004-10-27 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20040913

2001-01-31 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20001026

2001-01-31 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE