Global patent index - EP 1071812 A1

EP 1071812 A1 2001-01-31 - A METHOD, REAGENT SOLUTION AND KITS FOR DNA SEQUENCING

Title (en)

A METHOD, REAGENT SOLUTION AND KITS FOR DNA SEQUENCING

Title (de)

METHODE, REAGENZLÖSUNG UND KITS ZUR DNA-SEQUENZIERUNG

Title (fr)

TECHNIQUE, SOLUTION REACTIVE ET KITS POUR LE SEQUEN AGE DE L'ADN

Publication

EP 1071812 A1 (EN)

Application

EP 99904129 A

Priority

  • US 9901084 W
  • US 1238598 A

Abstract (en)

[origin: WO9937810A1] Solutions are disclosed which improve the efficiency of DNA sequencing reactions using substituent-labeled, e.g., dye-labeled ddNTPs, and thermostable DNA polymerase. The components of the solution include an effective concentration of manganese ion and a metal ion buffer compound. The use of such solutions is also described.

IPC 1-7 (main, further and additional classification)

C12Q 1/68; C07H 19/04; C07H 19/048; C12P 19/34

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); C07H 19/10 (2006.01); C07H 19/20 (2006.01); C07H 21/00 (2006.01); C12Q 1/68 (2006.01)

CPC (invention and additional information)

C07H 21/00 (2013.01); C07H 19/10 (2013.01); C07H 19/20 (2013.01); C12Q 1/6869 (2013.01)

Combination set (CPC)

C12Q 1/6869 + C12Q 2565/301 + C12Q 2535/101 + C12Q 2527/137

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9937810 A1 19990729; EP 1071812 A1 20010131; EP 1071812 A4 20020515; JP 2002510466 A 20020409

INPADOC legal status

2003-07-09 [18D] DEEMED TO BE WITHDRAWN

- Ref Legal Event Code: 18D

- Effective date: 20021217

2002-09-18 [17Q] FIRST EXAMINATION REPORT

- Ref Legal Event Code: 17Q

- Effective date: 20020806

2002-06-05 [RAP1] TRANSFER OF RIGHTS OF AN EP PUBLISHED APPLICATION

- Owner name: AMERSHAM BIOSCIENCES CORP.

- Ref Legal Event Code: RAP1

2002-05-15 [A4] SUPPLEMENTARY SEARCH REPORT

- Ref Legal Event Code: A4

- Effective date: 20020403

2002-05-15 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

2002-05-15 [RIC1] CLASSIFICATION (CORRECTION)

- Ref Legal Event Code: RIC1

- Free Format Text: 7C 12Q 1/68 A, 7C 12P 19/34 B, 7C 07H 19/04 B, 7C 07H 19/048 B, 7C 07H 21/00 B, 7C 07H 19/10 B, 7C 07H 19/20 B

2001-01-31 [17P] REQUEST FOR EXAMINATION FILED

- Ref Legal Event Code: 17P

- Effective date: 20000719

2001-01-31 [AK] DESIGNATED CONTRACTING STATES:

- Ref Legal Event Code: AK

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE