EP 1071812 A1 20010131 - A METHOD, REAGENT SOLUTION AND KITS FOR DNA SEQUENCING
Title (en)
A METHOD, REAGENT SOLUTION AND KITS FOR DNA SEQUENCING
Title (de)
METHODE, REAGENZLÖSUNG UND KITS ZUR DNA-SEQUENZIERUNG
Title (fr)
TECHNIQUE, SOLUTION REACTIVE ET KITS POUR LE SEQUEN AGE DE L'ADN
Publication
Application
Priority
- US 9901084 W 19990119
- US 1238598 A 19980123
Abstract (en)
[origin: WO9937810A1] Solutions are disclosed which improve the efficiency of DNA sequencing reactions using substituent-labeled, e.g., dye-labeled ddNTPs, and thermostable DNA polymerase. The components of the solution include an effective concentration of manganese ion and a metal ion buffer compound. The use of such solutions is also described.
IPC 1-7
IPC 8 full level
C07H 19/10 (2006.01); C07H 19/20 (2006.01); C07H 21/00 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6869 (2018.01)
CPC (source: EP)
C07H 19/10 (2013.01); C07H 19/20 (2013.01); C07H 21/00 (2013.01); C12Q 1/6869 (2013.01)
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9937810 A1 19990729; EP 1071812 A1 20010131; EP 1071812 A4 20020515; JP 2002510466 A 20020409
DOCDB simple family (application)
US 9901084 W 19990119; EP 99904129 A 19990119; JP 2000528716 A 19990119