Global Patent Index - EP 1071824 A2

EP 1071824 A2 2001-01-31 - QUANTITATIVE ANALYSIS OF GENE EXPRESSION

Title (en)

QUANTITATIVE ANALYSIS OF GENE EXPRESSION

Title (de)

QUANTITATIVE ANALYSE DER GENEXPRESSION

Title (fr)

ANALYSE QUANTITATIVE D'EXPRESSION DE GENE

Publication

EP 1071824 A2 (EN)

Application

EP 99921467 A

Priority

  • US 9908968 W
  • US 6567398 A

Abstract (en)

[origin: WO9954510A2] A method is provided for determining the level of expression of a gene of interest, or panel of genes of interest, in a biological sample. The method involves preparing a standard curve for each gene of interest by assaying a dilution series prepared from a total RNA in a reverse transcriptase-5'-exonuclease PCR amplification assay. A threshold cycle (Ct) for each member of the reverse transcribed-amplified is determined and plotted versus the log of the amount of total RNA in dilution series. The plot is used to determine an RNA equivalent from which the normalized RNA equivalent for the gene of interest in the sample is determined. The assay can be used to determine the effect of a treatment of the sample on the level of expression of a gene or interest or panel of genes of interest.

IPC 1-7 (main, further and additional classification)

C12Q 1/68

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); C12Q 1/68 (2006.01)

CPC (invention and additional information)

C12Q 1/686 (2013.01)

Combination set (CPC)

C12Q 1/686 + C12Q 2561/113 + C12Q 2545/114 + C12Q 2521/319

Citation (search report)

See references of WO 9954510A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9954510 A2 19991028; WO 9954510 A3 20000127; AU 3867199 A 19991108; CA 2325343 A1 19991028; EP 1071824 A2 20010131; IL 138851 D0 20011031; JP 2002512046 A 20020423

INPADOC legal status


2006-03-01 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20050727

2005-05-04 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20050316

2001-01-31 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20001102

2001-01-31 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE