EP 1073770 A2 20010207 - NOVEL METHOD FOR THE IDENTIFICATION OF CLONES CONFERRING A DESIRED BIOLOGICAL PROPERTY FROM AN EXPRESSION LIBRARY
Title (en)
NOVEL METHOD FOR THE IDENTIFICATION OF CLONES CONFERRING A DESIRED BIOLOGICAL PROPERTY FROM AN EXPRESSION LIBRARY
Title (de)
NEUARTIGES VERFAHREN ZUR IDENTIFIZIERUNG VON KLONEN MIT EINER GEWÜNSCHTEN BIOLOGISCHEN EIGENSCHAFT, AUSGEHEND VON EINER EXPRESSIONSGENBANK
Title (fr)
NOUVEAU PROCEDE PERMETTANT L'IDENTIFICATION DE CLONES CONFERANT UNE PROPRIETE BIOLOGIQUE DESIREE DANS UNE BANQUE D'EXPRESSION
Publication
Application
Priority
- EP 9902963 W 19990430
- US 7059098 A 19980430
Abstract (en)
[origin: US2010152051A1] The present invention relates to a novel method for the identification and/or characterization of clones conferring a desired biological property from an expression library. The method of the invention comprises the step of analyzing for the expression of at least one (poly)peptide, such as a tag expressed as a fusion protein, together with a recombinant insert of a clone of said expression library, wherein the clones of said expression library are arranged in arrayed form. Said (poly)peptide may be fused N-terminally or C-terminally to said insert. The method of the invention further comprises the steps of contacting a ligand specifically interacting with a (poly)peptide expressed by the insert of a clone conferring said desired biological property with a first replica of said library of clones in arrayed form and analyzing said library of clones for the occurrence of an interaction, and/or carrying out a hybridization or an oligonucleotide fingerprint with a nucleic acid probe specific for the insert of a clone conferring said desired biological property with a second replica of said library of clones arranged in arrayed form and analyzing said library of clones for the occurrence of a specific hybridization. Finally, the method of the invention requires the identification of clones wherein an expression of the at least one (poly)peptide in step (a) and/or an interaction in step (b) and/or a hybridization or an oligonucleotide fingerprint in step (c) can be detected. The present invention also relates to a kit useful for carrying out the method of the invention.
IPC 1-7
IPC 8 full level
G01N 33/50 (2006.01); C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12Q 1/68 (2018.01); G01N 33/15 (2006.01); C40B 40/06 (2006.01); C40B 60/14 (2006.01)
CPC (source: EP US)
C12N 15/1086 (2013.01 - EP US); C12Q 1/68 (2013.01 - EP US); B01J 2219/00387 (2013.01 - EP US); B01J 2219/00527 (2013.01 - EP US); B01J 2219/00585 (2013.01 - EP US); B01J 2219/0059 (2013.01 - EP US); B01J 2219/00596 (2013.01 - EP US); B01J 2219/00605 (2013.01 - EP US); B01J 2219/0061 (2013.01 - EP US); B01J 2219/00612 (2013.01 - EP US); B01J 2219/00637 (2013.01 - EP US); B01J 2219/00659 (2013.01 - EP US); B01J 2219/00707 (2013.01 - EP US); B01J 2219/00722 (2013.01 - EP US); C40B 40/06 (2013.01 - EP US); C40B 60/14 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI NL SE
DOCDB simple family (publication)
WO 9957311 A2 19991111; WO 9957311 A3 20000330; AT E282717 T1 20041215; AU 4136999 A 19991123; AU 770540 B2 20040226; CA 2326390 A1 19991111; DE 1073770 T1 20020704; DE 69921982 D1 20041223; DE 69921982 T2 20051229; EP 1073770 A2 20010207; EP 1073770 B1 20041117; JP 2002513588 A 20020514; JP 2010227109 A 20101014; JP 4540846 B2 20100908; US 2005244854 A1 20051103; US 2010152051 A1 20100617
DOCDB simple family (application)
EP 9902963 W 19990430; AT 99924858 T 19990430; AU 4136999 A 19990430; CA 2326390 A 19990430; DE 69921982 T 19990430; DE 99924858 T 19990430; EP 99924858 A 19990430; JP 2000547262 A 19990430; JP 2010110861 A 20100513; US 4517305 A 20050127; US 47381309 A 20090528