Global Patent Index - EP 1075550 A1

EP 1075550 A1 2001-02-14 - METHOD FOR IDENTIFYING AND REPRESENTING INTERINDIVIDUAL DIFFERENCES OF DNA SEQUENCES

Title (en)

METHOD FOR IDENTIFYING AND REPRESENTING INTERINDIVIDUAL DIFFERENCES OF DNA SEQUENCES

Title (de)

METHODE ZUR IDENTIFIZIERUNG UND DARSTELLUNG INTERINDIVIDUELLER DNA-SEQUENZUNTERSCHIEDE

Title (fr)

METHODE POUR L'IDENTIFICATION ET LA REPRESENTATION DE DIFFERENCES INTERINDIVIDUELLES DE SEQUENCES D'ADN

Publication

EP 1075550 A1 (DE)

Application

EP 99929095 A

Priority

  • DE 9901385 W
  • DE 19821114 A

Abstract (en)

[origin: DE19821114A1] The invention relates to a method for identifying and representing interindividual differences of DNA sequences. The invention is characterized in that; the genomic DNA of the individuals to be examined are isolated; segments of the genes to be compared, genomic regions, and genomes are defined; these segments are isolated in a targeted manner by means of PCR while using highly specific oligonucleotides and are produced in a sufficient number of copies; a simultaneous sequencing of all obtained gene segments is carried out, said gene segments now being available as PCR products; afterwards, the mixture of termination reactions which is produced thereby and which contains the individual sequences of all gene segments is electrophoretically separated on a sequencing gel; the mixture is transferred onto a membrane by means of direct transfer electrophoresis; afterwards, the individual sequences of a determined gene segment from the original mixture are made visible by hybridizing the membrane with a marked, highly specific oligonucleotide; this process is repeated unit all gene segments from the common pool are detected; afterwards, interindividual differences of DNA sequences are detected by image analysis, and a genetic profile model is established and evaluated by mathematical processing.

IPC 1-7 (main, further and additional classification)

C12Q 1/68

IPC 8 full level (invention and additional information)

G01N 33/53 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); G01N 27/447 (2006.01); G06F 17/30 (2006.01)

CPC (invention and additional information)

C12Q 1/6876 (2013.01)

Combination set (CPC)

C12Q 1/6876 + C12Q 2565/513 + C12Q 2565/125 + C12Q 2531/113

Citation (search report)

See references of WO 9957308A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

DE 19821114 A1 19991111; AU 4602199 A 19991123; CA 2340623 A1 19991111; EP 1075550 A1 20010214; JP 2002513915 A 20020514; WO 9957308 A1 19991111

INPADOC legal status


2003-06-18 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20021203

2001-02-14 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20001115

2001-02-14 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE