Global Patent Index - EP 1078044 A2

EP 1078044 A2 2001-02-28 - THROMBOLYTIC AGENTS DERIVED FROM STREPTOKINASE

Title (en)

THROMBOLYTIC AGENTS DERIVED FROM STREPTOKINASE

Title (de)

THROMBOLYTISCHE AGENTIEN AUS STREPTOKINASE

Title (fr)

AGENTS THROMBOLYTIQUES DERIVES DE STREPTOKINASE

Publication

EP 1078044 A2 (EN)

Application

EP 99924154 A

Priority

  • US 9910086 W
  • US 8439298 P

Abstract (en)

[origin: WO9957251A2] Structural information about the streptokinase-micro plasminogen complex has been used to identify the part of the streptokinase structure not involved in plasminogen complexation or activation. These nonessential portions can be modified to reduce antigenicity, for example, by changing the nonessential portions of streptokinose to more human-like polypeptide portions ("humanization of streptokinase"). One way this can be done is to compare the nonessential portion to a structural database of human proteins to find similar structures. Then the streptokinase nonessential structural part is replaced with the human structural part such as by genetic engineering of a mutant encoding the individual streptokinases, which is then expressed in a bacterial host such as E. coli. Alternatively, the nonessential portions can be removed or truncated to simplify streptokinase to a smaller molecule which retains plasminogen activation activity. Such smaller proteins should have reduced antigenicity and be cheaper and easier to produce. The modified streptokinases are useful in treating clotting disorders.

IPC 1-7 (main, further and additional classification)

C12N 9/00

IPC 8 full level (invention and additional information)

C12N 9/12 (2006.01); A61K 38/00 (2006.01); A61K 38/16 (2006.01); A61K 38/46 (2006.01); A61P 7/02 (2006.01); A61P 43/00 (2006.01); C07K 14/315 (2006.01)

CPC (invention and additional information)

A61K 38/166 (2013.01); C07K 14/3153 (2013.01); C07K 2319/00 (2013.01)

Citation (search report)

See references of WO 9957251A3

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9957251 A2 19991111; WO 9957251 A3 19991216; AU 4072399 A 19991123; CA 2327526 A1 19991111; EP 1078044 A2 20010228; JP 2002513810 A 20020514

INPADOC legal status


2007-12-14 [REG HK WD] APPLICATIONS WITHDRAWN, DEEMED TO BE WITHDRAWN, OR REFUSED AFTER PUBLICATION IN HONG KONG

- Document: HK 1033472

2002-11-27 [18W] WITHDRAWN

- Date of withdrawal: 20020930

2001-02-28 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20001206

2001-02-28 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE