EP 1078044 A2 20010228 - THROMBOLYTIC AGENTS DERIVED FROM STREPTOKINASE
Title (en)
THROMBOLYTIC AGENTS DERIVED FROM STREPTOKINASE
Title (de)
THROMBOLYTISCHE AGENTIEN AUS STREPTOKINASE
Title (fr)
AGENTS THROMBOLYTIQUES DERIVES DE STREPTOKINASE
Publication
Application
Priority
- US 9910086 W 19990506
- US 8439298 P 19980506
Abstract (en)
[origin: WO9957251A2] Structural information about the streptokinase-micro plasminogen complex has been used to identify the part of the streptokinase structure not involved in plasminogen complexation or activation. These nonessential portions can be modified to reduce antigenicity, for example, by changing the nonessential portions of streptokinose to more human-like polypeptide portions ("humanization of streptokinase"). One way this can be done is to compare the nonessential portion to a structural database of human proteins to find similar structures. Then the streptokinase nonessential structural part is replaced with the human structural part such as by genetic engineering of a mutant encoding the individual streptokinases, which is then expressed in a bacterial host such as E. coli. Alternatively, the nonessential portions can be removed or truncated to simplify streptokinase to a smaller molecule which retains plasminogen activation activity. Such smaller proteins should have reduced antigenicity and be cheaper and easier to produce. The modified streptokinases are useful in treating clotting disorders.
IPC 1-7
IPC 8 full level
A61K 38/00 (2006.01); A61K 38/16 (2006.01); A61K 38/46 (2006.01); A61P 7/02 (2006.01); A61P 43/00 (2006.01); C07K 14/315 (2006.01); C12N 9/12 (2006.01)
CPC (source: EP)
A61K 38/166 (2013.01); A61P 7/02 (2017.12); A61P 43/00 (2017.12); C07K 14/3153 (2013.01); C07K 2319/00 (2013.01)
Citation (search report)
See references of WO 9957251A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9957251 A2 19991111; WO 9957251 A3 19991216; AU 4072399 A 19991123; CA 2327526 A1 19991111; EP 1078044 A2 20010228; JP 2002513810 A 20020514
DOCDB simple family (application)
US 9910086 W 19990506; AU 4072399 A 19990506; CA 2327526 A 19990506; EP 99924154 A 19990506; JP 2000547207 A 19990506