EP 1078049 A1 20010228 - GENETICS-ASSISTED TARGET EVALUATION IN ANTIBACTERIAL DRUG DISCOVERY
Title (en)
GENETICS-ASSISTED TARGET EVALUATION IN ANTIBACTERIAL DRUG DISCOVERY
Title (de)
GENETISCH UNTERSTÜTZTE ZIELGENANALYSE ZUM AUFFINDEN ANTIBAKTERIELLER WIRKSTOFFE
Title (fr)
ESTIMATION DE CIBLES ASSISTEE GENETIQUEMENT ET UTILISEE DANS LA CONCEPTION DE MEDICAMENTS ANTIBACTERIENS
Publication
Application
Priority
- US 9910919 W 19990513
- US 8559398 P 19980515
Abstract (en)
[origin: WO9960111A1] The present invention provides a method, which is designated GATE (Genetics-Assisted Target Evaluation), for gene analysis in pathogenic bacteria. GATE functionally classifies genes according to their essential or nonessential character with respect to bacterial growth or pathogenicity. The technology uses a nonessential scorable gene as an internal standard against which the dispensability of a given target gene is assessed by gene disruption by Campbell-type integration. Functional analysis was performed on 26 genes in the Gram-positive pathogen Streptococcus pyogenes. Application of this method resulted in a clear identification of essential and nonessential loci: 16 of the 26 genes evaluated in S. pyogenes clearly encoded functions essential for bacterial growth, while the remaining 10 encoded functions that were nonessential. The successful application of GATE has also been demonstrated in Staphylococcus aureus.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12Q 1/04 (2006.01); C12Q 1/14 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6897 (2018.01)
CPC (source: EP)
C12Q 1/6897 (2013.01)
Citation (search report)
See references of WO 9960111A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9960111 A1 19991125; WO 9960111 A9 20000224; AU 4672099 A 19991206; CA 2328497 A1 19991125; EP 1078049 A1 20010228; JP 2002515242 A 20020528
DOCDB simple family (application)
US 9910919 W 19990513; AU 4672099 A 19990513; CA 2328497 A 19990513; EP 99930113 A 19990513; JP 2000549719 A 19990513