Global Patent Index - EP 1078049 A1

EP 1078049 A1 2001-02-28 - GENETICS-ASSISTED TARGET EVALUATION IN ANTIBACTERIAL DRUG DISCOVERY

Title (en)

GENETICS-ASSISTED TARGET EVALUATION IN ANTIBACTERIAL DRUG DISCOVERY

Title (de)

GENETISCH UNTERSTÜTZTE ZIELGENANALYSE ZUM AUFFINDEN ANTIBAKTERIELLER WIRKSTOFFE

Title (fr)

ESTIMATION DE CIBLES ASSISTEE GENETIQUEMENT ET UTILISEE DANS LA CONCEPTION DE MEDICAMENTS ANTIBACTERIENS

Publication

EP 1078049 A1 (EN)

Application

EP 99930113 A

Priority

  • US 9910919 W
  • US 8559398 P

Abstract (en)

[origin: WO9960111A1] The present invention provides a method, which is designated GATE (Genetics-Assisted Target Evaluation), for gene analysis in pathogenic bacteria. GATE functionally classifies genes according to their essential or nonessential character with respect to bacterial growth or pathogenicity. The technology uses a nonessential scorable gene as an internal standard against which the dispensability of a given target gene is assessed by gene disruption by Campbell-type integration. Functional analysis was performed on 26 genes in the Gram-positive pathogen Streptococcus pyogenes. Application of this method resulted in a clear identification of essential and nonessential loci: 16 of the 26 genes evaluated in S. pyogenes clearly encoded functions essential for bacterial growth, while the remaining 10 encoded functions that were nonessential. The successful application of GATE has also been demonstrated in Staphylococcus aureus.

IPC 1-7 (main, further and additional classification)

C12N 15/01; C12Q 1/04; C12Q 1/14; C12Q 1/68

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); C12Q 1/04 (2006.01); C12Q 1/14 (2006.01); C12Q 1/68 (2006.01)

CPC (invention and additional information)

C12Q 1/6897 (2013.01)

Citation (search report)

See references of WO 9960111A1

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9960111 A1 19991125; WO 9960111 A9 20000224; AU 4672099 A 19991206; CA 2328497 A1 19991125; EP 1078049 A1 20010228; JP 2002515242 A 20020528

INPADOC legal status


2004-06-09 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20031203

2001-02-28 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20001104

2001-02-28 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE