EP 1078080 A1 20010228 - METHODS FOR PRODUCING POLYPEPTIDES IN FILAMENTOUS FUNGAL MUTANT CELLS
Title (en)
METHODS FOR PRODUCING POLYPEPTIDES IN FILAMENTOUS FUNGAL MUTANT CELLS
Title (de)
VERFAHREN ZUM HERSTELLEN VON GENETISCH VERÄNDERTEN FILAMENTÖSEN PILZZELLEN
Title (fr)
METHODES DE PRODUCTION DE POLYPEPTIDES DANS DES CELLULES MUTANTES FONGIQUES FILAMENTEUSES
Publication
Application
Priority
- US 9910689 W 19990514
- US 7960198 A 19980515
- US 7934498 A 19980515
Abstract (en)
[origin: WO9960136A1] The present invention relates to methods for producing a polypeptide in enhanced amounts, comprising (a) cultivating a mutant of a parent filamentous fungal cell in a nutrient medium suitable for production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a modification of one or more second nucleic acid sequences encoding DDC2 and/or DDC3 polypeptides, or homologues thereof, and (ii) the mutant cell produces more of the polypeptide than the parent cell when cultured under the same conditions; and (b) recovering the polypeptide from the nutrient medium of the mutant cell. The present invention also relates to the second nucleic acid sequences, polypeptides encoded by the second nucleic acid sequences, and nucleic acid constructs, recombinant expression vectors, and host cells comprising the sequences. The present invention further relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells.
IPC 1-7
C12N 15/80; C12N 15/62; C12N 15/01; C12N 1/14; C12P 21/00; C07K 14/38; C12R 1/69; C12R 1/685
IPC 8 full level
C07K 14/38 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 9/00 (2006.01); C12N 9/30 (2006.01); C12N 9/36 (2006.01); C12N 15/09 (2006.01); C12N 15/80 (2006.01); C12P 21/00 (2006.01); C12P 21/02 (2006.01); C12R 1/19 (2006.01); C12R 1/685 (2006.01)
CPC (source: EP)
C12N 9/2462 (2013.01); C12N 15/80 (2013.01)
Citation (search report)
See references of WO 9960136A1
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI NL PT SE
DOCDB simple family (publication)
WO 9960136 A1 19991125; AU 4188099 A 19991206; CN 1301307 A 20010627; EP 1078080 A1 20010228; JP 2002515252 A 20020528
DOCDB simple family (application)
US 9910689 W 19990514; AU 4188099 A 19990514; CN 99806230 A 19990514; EP 99925633 A 19990514; JP 2000549742 A 19990514