Global Patent Index - EP 1078080 A1

EP 1078080 A1 2001-02-28 - METHODS FOR PRODUCING POLYPEPTIDES IN FILAMENTOUS FUNGAL MUTANT CELLS

Title (en)

METHODS FOR PRODUCING POLYPEPTIDES IN FILAMENTOUS FUNGAL MUTANT CELLS

Title (de)

VERFAHREN ZUM HERSTELLEN VON GENETISCH VERÄNDERTEN FILAMENTÖSEN PILZZELLEN

Title (fr)

METHODES DE PRODUCTION DE POLYPEPTIDES DANS DES CELLULES MUTANTES FONGIQUES FILAMENTEUSES

Publication

EP 1078080 A1 (EN)

Application

EP 99925633 A

Priority

  • US 9910689 W
  • US 7960198 A
  • US 7934498 A

Abstract (en)

[origin: WO9960136A1] The present invention relates to methods for producing a polypeptide in enhanced amounts, comprising (a) cultivating a mutant of a parent filamentous fungal cell in a nutrient medium suitable for production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a modification of one or more second nucleic acid sequences encoding DDC2 and/or DDC3 polypeptides, or homologues thereof, and (ii) the mutant cell produces more of the polypeptide than the parent cell when cultured under the same conditions; and (b) recovering the polypeptide from the nutrient medium of the mutant cell. The present invention also relates to the second nucleic acid sequences, polypeptides encoded by the second nucleic acid sequences, and nucleic acid constructs, recombinant expression vectors, and host cells comprising the sequences. The present invention further relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells.

IPC 1-7 (main, further and additional classification)

C12N 15/80; C12N 15/62; C12N 15/01; C12N 1/14; C12P 21/00; C07K 14/38; C12R 1/69; C12R 1/685

IPC 8 full level (invention and additional information)

C12N 15/09 (2006.01); C07K 14/38 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 9/00 (2006.01); C12N 9/30 (2006.01); C12N 9/36 (2006.01); C12N 15/80 (2006.01); C12P 21/00 (2006.01); C12P 21/02 (2006.01); C12R 1/19 (2006.01); C12R 1/685 (2006.01)

CPC (invention and additional information)

C12N 9/2462 (2013.01); C12N 15/80 (2013.01)

Designated contracting state (EPC)

AT BE CH DE DK ES FI FR GB GR IE IT LI NL PT SE

EPO simple patent family

WO 9960136 A1 19991125; AU 4188099 A 19991206; CN 1301307 A 20010627; EP 1078080 A1 20010228; JP 2002515252 A 20020528

INPADOC legal status


2004-08-11 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20040123

2003-10-29 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20030912

2001-11-21 [RAP1] TRANSFER OF RIGHTS OF AN EP PUBLISHED APPLICATION

- Owner name: NOVOZYMES BIOTECH, INC.

2001-11-21 [RAP1] TRANSFER OF RIGHTS OF AN EP PUBLISHED APPLICATION

- Owner name: NOVOZYMES A/S

2001-02-28 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20001113

2001-02-28 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH DE DK ES FI FR GB GR IE IT LI NL PT SE