EP 1079688 A1 20010307 - METHOD FOR EXPANDING PRIMATE B CELLS SELECTIVELY IN IMMUNOCOMPROMISED MICE AND PRODUCING LARGE NUMBERS OF ANTIGEN-SPECIFIC B LYMPHOCYTES FOR THE PRODUCTION OF PRIMATE MONOCLONAL ANTIBODIES
Title (en)
METHOD FOR EXPANDING PRIMATE B CELLS SELECTIVELY IN IMMUNOCOMPROMISED MICE AND PRODUCING LARGE NUMBERS OF ANTIGEN-SPECIFIC B LYMPHOCYTES FOR THE PRODUCTION OF PRIMATE MONOCLONAL ANTIBODIES
Title (de)
VERFAHREN ZUR EXPANSION VON PRIMAT B-ZELLEN SELEKTIV IN IMMUNITÄTSBEEINSTRÄCHTIGTEN MÄUSE UND PRODUKTION VON HOHER ANZAHL AN ANTIGENSPEZIFISCHE B-LYMPHOZYTEN FÜR DIE PRODUKTION VON PRIMAT MONOKLONALE ANTIKÖRPER
Title (fr)
PROCEDE PERMETTANT D'AUGMENTER DES LYMPHOCYTES B DE PRIMATES CHEZ LA SOURIS IMMUNODEPRIMEE, ET DE PRODUIRE DE GRANDS NOMBRES DE LYMPHOCYTES B SPECIFIQUES DE L'ANTIGENE POUR LA PRODUCTION D'ANTICORPS MONOCLONAUX DE PRIMATES
Publication
Application
Priority
- EP 99926411 A 19990525
- EP 9903605 W 19990525
- EP 98870121 A 19980526
Abstract (en)
[origin: WO9960846A1] The present invention relates to a method for reconstituting immunocompromised mice with primate cells, consisting of the following steps: (a) preparation of an immunocompromised mouse by administration of an antibody against the beta chain of the mouse interleukin 2 (IL-2) receptor, more particularly the monoclonal mouse TM- beta 1 monoclonal antibody and/or gamma irradiation, (b) intraspleen injection of primate cells into the spleen of the pre-treated immunocompromised mouse of step (a), where these primate cells preferably originate from a primate donor and where this primate donor may have been immunised with a well-defined antigen. This method was found to be highly suitable for expanding primate B lymphocytes in an animal model in a rapid, as yet unequalled manner. Other embodiments of this invention relate to immunocompromised mice obtained by the aforesaid method and also the use thereof as animal models, primate B cell culture produced and primate monoclonal antibodies derived therefrom.
IPC 1-7
IPC 8 full level
A01K 67/027 (2006.01); C07K 16/08 (2006.01); C07K 16/10 (2006.01); C07K 16/24 (2006.01); C12N 5/20 (2006.01); C12N 5/28 (2006.01); C12P 21/08 (2006.01); A61K 35/12 (2015.01)
CPC (source: EP)
A01K 67/0271 (2013.01); C07K 16/082 (2013.01); C07K 16/109 (2013.01); C07K 16/246 (2013.01); A61K 2035/124 (2013.01); C12N 2517/02 (2013.01)
Citation (search report)
See references of WO 9960846A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9960846 A1 19991202; AU 4368199 A 19991213; AU 751647 B2 20020822; CA 2332336 A1 19991202; EP 1079688 A1 20010307
DOCDB simple family (application)
EP 9903605 W 19990525; AU 4368199 A 19990525; CA 2332336 A 19990525; EP 99926411 A 19990525